稀少精子冷冻研究进展  

Current progress in cryopreservation of small numbers of sperm

作　　者：陈春华 沙艳伟 李萍 

机构地区：[1]厦门市妇幼保健院生殖医学中心,福建厦门361005

出　　处：《中华男科学杂志》2013年第8期753-757,共5页National Journal of Andrology

基　　金：福建省卫生厅青年科研课题(2010-2-108)~~

摘　　要：精子冷冻是人类辅助生殖领域中日益成熟的技术,但是常规精子冷冻方案却不适用于稀少精子冷冻,解决稀少精子冷冻技术将在很大程度上改善临床治疗少、弱精子症和无精子症患者的不育问题。寻找合适冷冻载体是目前稀少精子冷冻的研究重点之一,本文就近年来稀少精子不同载体冷冻方法:空卵膜载体冷冻法、微滴冷冻法、其他微型载体冷冻法和睾丸组织冷冻以及睾丸精子和附睾精子的冷冻效果作一综述。Human sperm cryopreservation is an increasingly mature technique in assisted reproduction. However, conventional sperm cryopreservation is not suitable for the cryopreservation of small numbers of sperm. The solution to the cryopreservation of small numbers of sperm may contribute a lot to the clinical treatment of asthenospermia, oligospermia and azoospermatism. Recently, many researchers focus on searching for appropriate carriers for the cryopreseivation of small numbers of sperm. This article outlines the effects of current cryopreservation methods including empty zona pellucida, microdrops, other mocrocarriers, testicular tissue cryopreservation and testicular sperm and epididymal sperm refrigeration.

关 键 词：精子冷冻 空卵膜 玻璃化冷冻 睾丸组织 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]