单侧隐睾症鞘膜及引带中INSL3和LGR8的表达和意义  

INSL3 and LGR8 expressions in perididymis and gubernaculum of patients with unilateral cryptorchidism

作　　者：宋非无[1] 李笃妙[1] 许雅丽[1] 吴强[1] 

机构地区：[1]福建医科大学附属第一医院小儿外科,福州350005

出　　处：《中华小儿外科杂志》2013年第8期599-602,共4页Chinese Journal of Pediatric Surgery

基　　金：福建省卫生厅青年科研课题（编号：2010-2-34）

摘　　要：目的探讨INSL3和LGR8在单侧隐睾症患儿睾丸鞘膜及引带中的表达和临床意义。方法采用RT-PCR技术检测INSL和LGR8在42例单侧隐睾儿童的睾丸鞘膜、引带（其中高位4例，中位31例，低位7例）和40例鞘膜积液儿童睾丸鞘膜和40例女性腹股沟斜疝疝囊的mRNA转录情况。结果INSL3和LGR8在不同位置单侧隐睾症患儿的鞘膜及引带中的表达差异无统计学意义（P〉O．05）；INSL3和LGR8在单侧隐睾患儿引带的表达低于它们在隐睾患儿睾丸鞘膜的表达（P〈0．05）；INSL3和LGR8在单侧隐睾睾丸鞘膜的表达低于睾丸鞘膜积液儿童睾丸鞘膜的表达（P〈0．05）；INSL3和LGR8在女性疝囊中的表达水平低于睾丸鞘膜积液儿童的睾丸鞘膜的表达（P〈0．05）。结论INSL3和LGR8在不同位置单侧隐睾症患儿的鞘膜及引带中的表达差异无统计学意义，提示INSL3和LGR8在隐睾的发生过程不同阶段可能均起作用；INSL3和LGR8在单侧隐睾症儿童睾丸引带中低表达，可能是导致隐睾症的病因之一。Objective To investigate the expression levels and the clinical significance of INSL3 and LGR8 in patients with unilateral eryptorchidisrrL Methods The expression levels of INSL3 and LGR8 were evaluated using RT-RCR in gubernaculum and perididymis in 42 unilateral eryptorehidism （high position 4 cases; middle 31 cases; low 7 cases）. Controls were taken from 40 perididymis in 40 hydrocele patients and 40 hernia sacs in female indirect inguinal hernia patients. Results The expres- sion levels of INSL3 and LGR8 in perididymis tissues and gubernaculums of different type of unilateral cryptorchidism showed no significant difference （P〈0. 05）. The expression levels of INSL3 and LGR8 in gubernaculums were lower than those in perididymis tissues （P〈0. 05） ; and the expressions of IN- SL3 and LGR8 in perididymis tissues in unilateral cryptorchidism were lower than those in hydrocele testis （P〈0. 05）. The expressions of INSL3 and LGR8 in hernia sac of female indirect inguinal hernia were lower than those in perididymis of hydrocele testis （P〈0. 05）. Conclusions The expression lev els of INSL3 and LGR8 in perididymis tissues and guhernaculums of different type of unilateral eryp- torchidism have no significant difference. It would imply that INSL3 and LGR8 might have a role in different stages of testicular descent. Lower levels of INSL3 and LGR8 may be associated with altered ~ubernacular develoDment and crvDtorehidism.

关 键 词：胰岛素样激素3 受体 G-蛋白偶联 隐睾 

分 类 号：R726.9[医药卫生—儿科]