严重烫伤大鼠胰岛β细胞结构和功能的变化  

Structural and functional changes in islet beta cells in severely scalded rats

作　　者：李大伟[1] 申传安[1] 柴家科[1] 马丽[1] 尚玉茹[1] 李龙珠[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100048

出　　处：《中华烧伤杂志》2013年第4期355-359,共5页Chinese Journal of Burns

基　　金：国家自然科学基金面上项目（30971128）;全军医学科技“十二五”科研面上项目（CWS11J097）;首都I临床特色应用研究项目（Z12110700102092）

摘　　要：目的观察严重烫伤大鼠胰岛B细胞的结构和功能变化，探讨与糖代谢障碍之间的关系。方法将72只Wistar大鼠按随机数字表法分为烫伤组和假伤组，每组36只。将烫伤组大鼠背部浸于94℃热水12s、腹部6S，造成50％TBSAⅢ度烫伤；假伤组大鼠背部、腹部浸于37℃温水模拟烫伤。伤后6h及3、7d，各组分别取12只大鼠行腹腔葡萄糖耐量试验（IPGTT），检测血糖水平并计算血糖曲线下面积（AUC）。IPGTT结束后，分离大鼠胰腺组织行胰岛素免疫荧光和细胞核染色，观测胰岛内胰岛素阳性表达面积比、13细胞面积和数量（简称“胰岛内3项指标”）。对数据行重复测量方差分析及析因设计方差分析，两两比较采用LSD法。结果（1）伤后6h和3d，烫伤组大鼠葡萄糖注射前后血糖水平总体及各时相点血糖水平均显著高于假伤组（处理因素主效应，值分别为79．372、32．962，P值均小于0．001；两两比较，P〈0．05或P〈0．01）。伤后7d，2组大鼠葡萄糖注射前后血糖水平总体及各时相点血糖水平均相近（P值均大于0．05）。（2）烫伤组大鼠血糖AUC总体明显高于假伤组（处理因素主效应，F：337．87，P〈0．01）。伤后6h及3d，与假伤组[（1019±32）、（1003±72）mmol·min·L-1]比较，烫伤组大鼠血糖AUC均有显著升高[（1501±163）、（1132±67）mmol·min·L-1，P值均小于0．001]。伤后7d，2组大鼠的血糖AUC相近（P〉0．05）。烫伤组大鼠伤后3、7d血糖AUC均显著低于伤后6h（P值均小于0．001）。（3）烫伤组大鼠胰岛内3项指标水平总体均明显低于假伤组（处理因素主效应，值分别为135．17、24．75和39．35，P值均小于0．01）。2组大鼠伤后6h胰岛内3项指标水平无明显差异（P值均大于0．05），而烫伤组大鼠伤后3、7d胰岛内3项指标水平[0．47±0．05、0．51±0。07，（0．032±0．008）、（0．037±0．008）mm2，（303Objective To observe the structural and functional changes in islet beta cells in severe- ly scalded rats, and to explore its relationship with dysfunction of glyeometabolism. Methods Seventy-two Wistar rats were divided into scald （S） group and sham injury （SI） group according to the random number table, with 36 rats in each group. Rats in group S were inflicted with 50% TBSA full-thickness scald by a 12-s immersion of back and a 6-s immersion of abdomen in 94 ℃ hot water. Rats in group SI were sham in- jured through immersion of back and abdomen in 37 ℃ warm water. At post injury hour （PIH） 6 and on post injury day （PID） 3 and 7, plasma glucose level was measured for intraperitoneal glucose tolerance test （IPGTT） in 12 rats of each group, and the area under the curve （AUC） of plasma glucose level was calcu- lated. After the IPGTT, pancreatic tissue was harvested and subjected to a double immunostaining for insulin and cell nuclei to determine the pancreatic insulin-positive area ratio, and the area and number of beta cells in the islets （ referred to as ＂the three indicators in the islets＂ ）. Data were processed with the analysis of re- peated measures and factorial designed analysis of variance, and LSD test was applied for paired comparison. Results （1） At PIH 6 and on PID 3, the overall plasma glucose levels of rats in group S before and after injection of glucose and at each time point were obviously higher than those of rats in group SI （ with F values of main effects respectively 79. 372 and 32. 962, P values all below 0. 001 ; with P values of paired compari- son below 0.05 or 0.01 ）. On PID 7, the overall plasma glucose levels in the two groups before and after in- jection of glucose and at each time point were close （ with P values all above 0.05 ）. （2） The overall AUC of plasma glucose levels of rats in group S was higher than that of rats in group SI （ main effects ： F = 337.87, P 〈0.01）. Compared with those of rats in group SI [（1019±32�

关 键 词：烧伤 胰腺 糖代谢 高糖血症 

分 类 号：R644[医药卫生—外科学]