神经生长因子对糖尿病神经病变大鼠神经肽和神经传导速度的影响  

Influence of nerve growth factor in neuropeptides and nerve conduction velocity in rats with experimental diabetic peripheral neuropathy

作　　者：卫重娟[1] 程焱[2] 梁浩[2] 陈英[2] 孔晓冬[1] 古今颖[1] 

机构地区：[1]天津医科大学总医院干部保健部、天津市老年病学研究所,天津300052 [2]天津医科大学总医院干部保健部、天津市老年病学研究所神经内科.天津市神经病学研究所.“211重点工程学科”,天津300052

出　　处：《中华神经医学杂志》2013年第8期779-782,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨神经生长因子对糖尿病周围神经病变大鼠神经肽和神经传导速度的影响. 方法 雄性Wistar大鼠35只按随机数字表法分为健康对照组（n=10）、糖尿病模型组（n=13）和神经生长因子治疗组（n=12）,后两组用链脲佐菌素制成糖尿病周围神经病变大鼠模型,并给予神经生长因子治疗组神经生长因子治疗（40μg/kg）.显微镜下观察并计算背根神经节中P物质、降钙素基因相关肽（CGRP）免疫阳性细胞率,检测运动神经传导速度（MNCV）和感觉神经传导速度（SNCV）. 结果 糖尿病模型组大鼠背根神经节中P物质、CGRP免疫阳性细胞率（27.710％±3.471％；36.360％±12.027％）以及神经生长因子治疗组治疗前MNCV [（35.80±6.19） m/s]、SNCV[（39.62±6.69） m/s]与健康对照组[P物质：44.225％±8.213％；CGRP：47.400％±13.723％；MNCV：（55.83±10.30） m/s; SNCV：（47.02±7.52） m/s]相比显著下降,差异有统计学意义（P＜0.05）.经神经生长因子治疗后,P物质、CGRP免疫阳性细胞率（49.417％±6.753％;53.811％±7.125％）较糖尿病模型组显著增高,MNCV[（41.80±3.45） m/s]、SNCV[（42.92±6.69） m/s]均治疗前显著增高,差异有统计学意义（P＜0.05）. 结论 糖尿病周围神经病变大鼠可出现神经传导速度下降和神经生长因子相关神经肽P物质、CGRP缺乏,而神经生长因子可促进神经肽的表达并提高神经传导速度.Objective To investigate the influence of nerve growth factor （NGF） in neuropeptides and nerve conduction velocity in rats with experimental diabetic peripheral neuropathy （DNP）.Methods Thirty-five 6-week-old male Wistar rats,weighing 175-215 g,were chosen in our study and randomly divided into healthy control group （n=10）,DNP model group （n=13） and NGF treatment group （n=12）.Rats in the latter 2 group were induced with the interference of administered streptozotocin,and rats in the NGF treatment group were treated with NGF （40 μg/kg）.The immunopositive cells percentages of substance P （SP） and calcitonin gene-related peptide （CGRP） in dorsal root ganglion （DRG） were observed and measured; the motor nerve conduction velocity （MNCV） and sensory nerve conduction velocity （SNCV） were measured and compared among the three groups.Results As compared with those in the healthy control group,the immunopositive cells percentages of SP （27.710%±3.471% vs 44.225%±8.213%） and CGRP （36.360%±12.027% vs 47.400%±13.723%） in the DRG of DNP model group were significantly decreased （P〈0.05）; as compared with those in the healthy control group,the MNCV[（35.80±6.19） m/s vs （55.83±10.30） m/s] and SNCV[（39.62±6.69） m/s vs （47.02 ±7.52） m/s）] of DNP model group were significantly decreased （P〈0.05）; however,obvious increase of SP （49.417%±6.753%） and CGRP （53.811%±7.125%）,MNCV （41.80±3.45） m/s and SNCV （42.92±6.69 m/s） in the NGF treatment group was noted as compared with those in the DNP model group （P〈0.05）.Conclusion The nerve conduction velocity and NGF related neuropeptides （SP and CGRP） decrease in rats with DNP; treatment with NGF promotes the expression ofneuropeptides and increases the nerve conduction velocity.

关 键 词：糖尿病周围神经病变 神经生长因子 神经肽 神经传导速度 

分 类 号：R587.2[医药卫生—内分泌]