自主运动可促进大鼠下丘脑神经祖细胞的增殖与分化(英文)  

Voluntary exercise promotes proliferation and differentiation of adult rat hypothalamus progenitor cells

作　　者：李光华[1] 松崎健太郎 王银[1] 赵楠[1] 杨敏[1] 紫藤治 

机构地区：[1]宁夏医科大学生理学系,宁夏银川750004 [2]岛根大学医学部环境生理学教研室,日本出云693-8501

出　　处：《南方医科大学学报》2013年第8期1099-1106,共8页Journal of Southern Medical University

基　　金：Supported by National Natural Science Foundation of China(81060230);Ningxia Natural Science Foundation(NZ10111)~~

摘　　要：目的探讨自主运动对大鼠下丘脑神经细胞增殖与分化的影响。方法雄性Wistar大鼠,随机分为对照组(SE)和自主运动组(EX),以上两组又进一步分为6、13、23、33、43、53 d共6个亚组。各组大鼠从实验开始,连续5 d腹腔注射BrdU,于上述时间点处死大鼠并取脑组织观察下丘脑的BrdU阳性细胞数的变化。结果免疫组化结果表明,EX组下丘脑的BrdU阳性细胞数在23、33、43、53 d亚组中明显高于SE组(P<0.05)。EX组中经43 d的自主运动后,BrdU阳性细胞数与神经元双染色细胞数明显增加(P<0.05),对照组各亚组则无显著性变化。结论自主运动可促进下丘脑的神经祖细胞增殖并分化为神经元,这可能与长期的运动锻炼有一定关系。Objective To investigate the effect of voluntary exercise on the proliferation and differentiation of hypothalamus progenitor cells in adult rats.Methods Male Wistar rats were divided into voluntary exercise(EX) and sedentary(SE) groups,both of which were further divided into 6 subgroups for observation on days 6,13,23,33,43 and 53.Bromodeoxyuridine(BrdU) was intraperitoneally injected daily for 5 consecutive days after commencing voluntary exercise,and at the specified time points during voluntary exercise,the rats' brains were removed to observe the numbers of BrdU-positive cells in the hypothalamus.Results Immunohistochemical analysis showed that the numbers of BrdU-positive cells in the hypothalamus of EX subgroups were significantly greater than those of SE subgroups on days 23,33,43 and 53.In EX group,the number of BrdU-positive cells double-stained for a mature neuron marker increased after 43 days of voluntary exercise,which did not occur in SE group.Conclusion Long-term voluntary exercise can promote the proliferation of neuronal progenitor cells in the hypothalamus and their differentiation into neurons.

关 键 词：下丘脑 自主运动 祖细胞 大鼠 

分 类 号：G804[文化科学—运动人体科学]