芹菜素对人肺癌NCI-H460细胞增殖及凋亡的影响  

Effect of apigenin on proliferation and apoptosis of human lung cancer NCI-H460 cells

作　　者：潘雪刁[1] 杨周萍[1] 杨志英[2] 周四桂[1] 张海涛[1] 臧林泉[1,3] 

机构地区：[1]广东药学院药科学院药理系及新药筛选与药效学评价中心,广东广州510006 [2]深圳市第二人民医院营养科,广东深圳518035 [3]广东药学院广东省分子流行病学重点实验室,广东广州510310

出　　处：《南方医科大学学报》2013年第8期1137-1140,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81102465);国家科技重大专项"重大新药创制"项目(2011zx09102-001-31);广东省科技计划项目(2010B031500034);广州市科技计划项目(2010J-E091)~~

摘　　要：目的研究芹菜素对人肺癌NCI-H460细胞增殖及凋亡的影响。方法应用10~80μmol/L芹菜素处理NCI-H460细胞,MTT法检测细胞增殖抑制率;Hoechst 33258细胞核染色法观察细胞凋亡的形态学改变;流式细胞仪AnnexinV-FITC/PI双染色法检测细胞凋亡率;Western blotting检测凋亡相关信号分子Bax、Bcl-2和caspase-3的蛋白表达水平。结果芹菜素对NCI-H460细胞的增殖抑制作用呈浓度和时间依赖性;芹菜素处理组细胞出现核固缩、染色质凝集和核碎片化等典型的细胞凋亡特征;NCI-H460细胞凋亡率随着芹菜素浓度增加逐渐增高;芹菜素处理组细胞Bax和caspase-3蛋白表达增加,Bcl-2蛋白表达降低。结论芹菜素能够抑制人肺癌NCI-H460细胞的增殖,并诱导其凋亡,其机制可能与上调Bax和下调Bcl-2的表达,导致Bcl-2/Bax比值下降,caspase-3活化有关。Objective To study the effect of apigenin on the proliferation and apoptosis of human lung cancer cell line NCIH460.Methods NCI-H460 cells were cultured with different concentrations of apigenin,and MTT assay was used to evaluate the cell inhibition rates.Apoptosis of NCI-H460 cells was observed under a fluorescence microscope with Hoechst 33258 staining and quantified by flow cytometry using annexin V-FITC/PI stain.The expressions of apoptosis-related proteins Bax,Bcl-2 and caspase-3 were analyzed by Western blotting.Results Apigenin causes concentration-and time-dependent inhibition of the proliferation of the cells.NCI-H460 cells treated with apigenin showed significant morphological changes of apoptosis,and the cell apoptotic rates increased as apigenin concentration increased.Western blotting demonstrated that apigenin increased the protein levels of Bax and caspase-3 and reduced the protein expression of Bcl-2.Conclusion Apigenin can inhibit the proliferation and induce apoptosis of NCI-H460 cells possibly by up-regulating expression of Bax and caspase-3 and downregulating the expression of Bcl-2.

关 键 词：芹菜素 肺癌 细胞增殖 细胞凋亡 BAX BCL-2 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]