牙釉质结合肽的淘选及亲和力分析的实验研究  

Biopanning and affinity analysis of enamel binding peptides

作　　者：魏蔚[1] 彭周[2] 邓捷[1] 赵婷[1] 张威[1] 毛靖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔正畸科,湖北武汉430030 [2]华中科技大学材料科学与工程学院,湖北武汉430074

出　　处：《临床口腔医学杂志》2013年第8期451-453,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(NSFC30870651;NSFC30970751)

摘　　要：目的:利用噬菌体表面随机肽展示技术淘选获得牙釉质结合肽。方法:以经过预处理的牙釉质片为模板,通过四轮淘选获得牙釉质结合肽的富集。从第3轮和第4轮产物中分离出总计20条单克隆进行测序及亲和力分析。结果:产物中噬菌体滴度在第2轮产物中得到最大富集之后逐渐下降。所测序的20个单克隆中没有发现共有序列。其中有4个单克隆具有明显的强于WM13噬菌体的牙釉质结合力。结论:四个序列被认为具有较强的牙釉质结合能力。所测序列中未发现明显的共有序列。Objective： To select and identify a subset of enamel binding peptides using phage display library. Method： A piece of pretreated enamel was served as substrate in biopanning enamel binding peptides. After four rounds panning, tweenty clones isolated from the 3rd and 4th round were sequenced and tested for binding affinity. Result： the titer of plaque forming units in subset of each panning rounds reached the peak at 2nd round and decreased with the further panning rounds. Four out of tweenty clones were found to have higher binding affinity than wild M13 phage. Conclusion： Four clones were identified to be strong binder for enamel. And no repeated sequences were found in the present research.

关 键 词：牙釉质 结合肽 噬菌体展示 亲和力分析 

分 类 号：Q812[生物学—生物工程]