MR分子成像评价壁虎活性单体对人肺腺癌SPC细胞的作用  

MR molecular imaging in evaluation on the effect of Gecko swinhonis active monomer on human lung adenocarcinoma SPC cells in vitro

作　　者：程鑫[1] 张兆光[1] 韩明[1] 赵荣荣[1] 赵瑶[1] 张仕状[1] 

机构地区：[1]潍坊医学院附属医院影像中心潍坊医学院医学影像学系,山东潍坊261031

出　　处：《中国医学影像技术》2013年第8期1253-1257,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(81102735;81202992;81072977;30772716);山东省自然科学基金(ZR2011HQ023);山东省卫生厅科技计划(2011QZ026);潍坊市科技局资助项目(201002045;2010079;2010080)

摘　　要：目的利用前期制备的超微超顺磁性氧化铁(USPIO)标记的含有精氨酸-甘氨酸-天冬氨酸(RGD)序列的环肽探针(RGD-USPIO分子探针)进行MR成像,评价壁虎活性单体BH1273209对人肺腺癌SPC细胞的作用效果。方法将SPC细胞接种于6孔板中培养24h,分为实验组和对照组,分别加入1mg/ml的BH1273209和培养液,培养48h后加入含铁浓度25μg/ml的RGD-USPIO培养1h,进行普鲁士蓝染色。将细胞重悬于1%琼脂糖凝胶中,置于Eppendorf管内进行MR扫描。结果随浓度增加,BH1273209对SPC细胞的抑制作用增强,1 mg/ml时抑瘤率最高;铁浓度为25μg/ml时RGD-USPIO探针对SPC细胞活性无明显影响。对照组SPC细胞内可见较多蓝染颗粒,实验组SPC细胞内未见明显的蓝染颗粒。T2WI中,实验组的SNR(132.26±17.24)较对照组(4.89±3.35)明显增高(P<0.01)。结论MR分子成像可简便、准确地评价BH1273209对SPC细胞的作用效果;BH1273209使SPC细胞结合RGD-USPIO明显减少,推测其通过抑制整合素配体-受体结合而发挥抗肿瘤作用。Objective To proceed molecular MRI with RGD sequence-ultrasmall superparamagnetic iron oxid （RGD-USPIO） prepared previously, and to evaluate the effect of Gecko swinhonis active monomer BH1273209 on human lung adenocarcinoma SPC cell. Methods The effect of different concentrations of BH1273209 and RGD-USPIO on SPC cells by MTT assay was surveyed. Then the optimum densities of BH1273209 and RGD-USPIO were groped to base for experiment in vitro. SPC cells were cultured 24 h in 6-well plates conventionally, and divided into experimental group and control group, in which BH1273209 of 1 mg/ml and 1640 medium was added to SPC cells respectively. The cells were cocultured for 48 h, then RGD-USPIO with 25 μgFe/ml was added to cells and cocultured for 1 h. The cells were resuspended in 1% agarose gel placed in Eppendorf tube, and proceeded MR scanning. Results Inhibitory effect of BH1273209 on SPC cells raised with the concentration of BH1273209 increased. The inhibitory effect of BH1273209 of 1 mg/ml was the maximum. There was no effect of RGD-USPIO probe with 25 μgFe/ml on SPC cells activity. There were many blue-stain grana in SPC cells of control group, but no blue-stain grana in SPC cells of experimental group. SNR of experimental group （132.2617.24） was significantly higher than that of control group （4.893.35, P〈0.01）. Conclusion MR molecular imaging can conveniently and accurately evaluate the anti-tumor effect of BH1273209 on SPC cells. RGD-USPIO molecular probe combined with SPC cells is lessened after adminiatration of BH1273209, speculating that BH1273209 may develop anti-tumor effect through inhibit the binding of integrin ligand and its receptor.

关 键 词：壁虎活性单体 分子探针 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R332[医药卫生—诊断学]