茶皂素与四种抗菌药物的体外联合抗菌效果研究  

Experimental Bacteriostasis of Tea Saponin Combining with Four Antimicrobials in Vitro

作　　者：潘任桃[1] 卢素云[1] 刘兆颖[1] 孙志良[1] 

机构地区：[1]湖南农业大学动物医学院国家植物功能成分利用工程技术研究中心生物兽药分中心,湖南长沙410128

出　　处：《动物医学进展》2013年第8期119-122,共4页Progress In Veterinary Medicine

基　　金：湖南省科技重大专项(2012FJ1004-3)

摘　　要：为探究茶皂素与其他抗菌药物对大肠埃希菌、金黄色葡萄球菌的体外联合抗菌效果,采用琼脂稀释法、多孔板-MTT比色法和棋盘法,分别测定了茶皂素与庆大霉素、氟苯尼考、恩诺沙星、氨苄青霉素对大肠埃希菌、金黄色葡萄球菌的最小抑菌浓度(MIC)及分级抑菌浓度(FIC)指数。结果显示多孔板-MTT比色法与琼脂稀释法结果一致率为87.5%;联合使用中,茶皂素与4种抗菌药物都表现为相加作用。In ordle to explore the experimental bacteriostasis of tea saponin with antimicrobials against E. coli and Staphylococcus aureus in vitro ,the classical agar dilution method, microplate-MTT colorimetric method and checkerboard method were used. The experimental bacteriostasis of tea saponin with gentami- cin, florfenicol,enrofloxacin and ampicillin against E. coli and Staphylococcus aureus were tested in vitro, and the minimum inhibitory concentration（MIC） and the fractional inhibitory concentration（FIC） were cal- culated. The experiment showed that the coincidence rates between microplate-MTT colorimetric method and easier than classical agar dilution method were 87.5 % ,and microplate-MTT colorimetric method was easier than classical agar dilution method. From the combination test, tea saponin with the four antimicrobi- als had additive actions.

关 键 词：茶皂素 联合抗菌 MTT 最小抑菌浓度 分级抑菌浓度 

分 类 号：O629.13[理学—有机化学] S859.796[理学—化学]