马立克病鸡血清生化指标的检测与分析  

Detection and Analysis of Biochemical Indexes in Serum of Chickens with Marek's Disease

作　　者：田文霞[1] 王颖[1] 刘红霞[1] 喻进[1] 宁官保[1] 高荣琨[1] 高文伟[1] 赵宇军[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《动物医学进展》2013年第8期127-130,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31072179);山西省自然科学基金项目(2009011043-1);山西省科技攻关项目(20130311027-3);山西省高等学校大学生创新创业训练项目(2011088);山西农业大学第九批大学生科技创新项目(9-007;9-011)

摘　　要：研究鸡马立克病对血清生化指标的影响,将72只1日龄海兰褐公雏鸡随机分成对照组(C)与攻毒组(V),C组每只鸡腹腔注射0.2mL灭菌Hanks液,V组每只鸡腹腔注射用灭菌Hank’s液稀释20倍的马立克病毒强毒京-1株血毒稀释液0.2mL。分别于3、6、10、15、20、26、35、45、60d对2组各取4只鸡进行心脏采血,剖检,观察病理变化,析出血清,分别采用IFCC速率法、酶法和直接法测定血清中γ-谷氨酰转肽酶(GGT)活性、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的含量变化。结果显示,攻毒后血清中GGT活性在35d时明显高于对照组,而其他阶段偏低,且差异不明显;HDL含量低于对照组,15d时差异极显著(P<0.01);LDL含量高于对照组,且15d时差异极显著(P<0.01)。由此提示,鸡血清GGT,HDL与LDL含量变化可作为预测马立克病的重要指标。In this study, we investigated seral biochemical indexes in Marek＇s disease （MD） chickens. 72 one day old Hy-Line Brown male chickens were randomly divided into a control group （C） and an experimental group challenged with Jing-1 strain（V）. In the virus-challenged group （V）, each chick was injected with 0.2 mL of solution obtained from 20 times diluting Jing-1 strain with sterile Hanks solution. In the group C,each chick was given with 0.2 mL of sterile Hanks solution. All chicks were kept in accordance with normal breeding conditions and the incidence of MD was observed. On day 3,6,10,15,20,26,35,45 and 60 after inoculation, four chicks from each group were sampled for blood from heart, and then were necropsied when they were sacrificed by cervical dislocation after ether anaesthesia. The activity of gamma glutamyl- transferase （GGT）,the content of high density lipoprotein （HDL） and low density lipoprotein （LDL） were detected hy IFCC velocity method,enzymic method and direct method respectively. Experimental results

关 键 词：马立克病 动脉粥样硬化 Γ-谷氨酰转肽酶 高密度脂蛋白 低密度脂蛋白 

分 类 号：S852.659.1[农业科学—基础兽医学] S858.31[农业科学—兽医学]