利用双向电泳构建并优化超数排卵犊牛卵泡液蛋白质图谱  

Proteomic map construction and optimization of calf follicular fluids by two dimensional gel electrophoresis

作　　者：赵海峰[1] 张飒[1] 陈集成[1] 柯浩[1] 陈东峰[1] 陈宝剑[1] 陆凤花[1] 韦英明[1] 

机构地区：[1]广西大学动物繁殖研究所/亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

出　　处：《广东农业科学》2013年第14期123-125,共3页Guangdong Agricultural Sciences

基　　金：广西自然科学基金(2013GXNSFAA019095);广西科技攻关项目(10100007-4)

摘　　要：以2～5月龄超数排卵犊牛卵泡液为研究对象,比较不同pH值的IPG胶条和4种上样量(150、250、350、450μg)对卵泡液蛋白图谱构建的影响。结果表明,在pH 4～7的IPG胶条、上样量为350μg时,获得较好质量的卵泡液蛋白图谱,并获得约319个蛋白点。成功构建的卵泡液蛋白双向电泳技术,为进一步研究犊牛与成年牛卵泡液中差异蛋白质组学分析奠定基础。In order to estabilish and optimize a two dimensional gel electrophoresis （2-DE）technique for proteome analysis of calf follicular fluid, effects of two different IPG strips and 4 kinds of loading volumes （150 μg, 250 μg, 350 μg, 450 μg） on the quanty of the 2-D maps were studied. The results showed that one perfect and clearly 2-DE map was obtained when pH 4-7 IPG strip with 24 cm and loading volume of 350μg were used. About 319 spots in the 2-DE map were detected by the Image Master 2-D platinum software. Our established 2-DE technique of calf follicular fluids can be effectively applied in different peoteins between calves and adult cattles.

关 键 词：双向电泳 蛋白质组学 犊牛 卵泡液 

分 类 号：S823[农业科学—畜牧学]