紫竹不同栽培类型PPO基因片段克隆及其SNP分析  

Cloning and SNP analysis of PPO genes with cultivars of Phyllostachys nigra

作　　者：肖国辉[1] 王弋[1] 娄永峰[1] 林新春[1] 郭小勤[1] 方伟[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《浙江农林大学学报》2013年第4期511-516,共6页Journal of Zhejiang A&F University

基　　金：浙江省科学技术攻关项目(2007C22081);浙江农林大学科研发展基金面上资助项目(2007FR025)

摘　　要：根据已报道的禾本科Poaceae植物PPO基因的保守序列(CuA和CuB区)设计1对引物,分别从紫竹Phyl-lostachys nigra 9个不同栽培变型中克隆到PPO基因片段。序列分析显示:9个序列彼此间核苷酸水平上一致性达到92.35%,氨基酸水平上的一致性达到81.83%。在某些栽培类型,一年紫和沟槽紫,该基因存在提前终止的现象。所获得的9条序列间存在明显的单核苷酸多态性(SNP)位点,多数位点引起非同义突变。根据所测得的序列对紫竹不同栽培类型的亲缘关系进行了遗传多样性分析。Polyphenol oxidase（PPO） fragments were cloned from cultivars of Phyllostachys nigra using a pair of primers designed according to a conserved region（CuA and CuB） of a PPO gene sequence in Poaceae family and analyzed with a sequence analysis.The genetic relationship of cultivars was also analyzed based on these fragments using DNAMAN software.Results of the sequence analysis showed that identity was 92.35% in nucleotide acid and 81.83% in amino acid.A truncated protein of the gene was found in some cultivars of P.nigra,such as’One-year-purple’and’Purple Groove’.Single-nucleotide polymorphism（SNP） sites were also found among these sequences.This could lead to non-synonymous mutations

关 键 词：植物学 紫竹 PPO基因 单核苷酸多态性(SNP) 遗传多样性 

分 类 号：S722[农业科学—林木遗传育种]