化学发光技术检测乙肝前S1蛋白的临床价值  

Clinical value of the detection of pre-S1 protein of hepatitis B virus with chemiluminescent assay

作　　者：顾春瑜[1] 许琼[1] 赵乔妹[1] 

机构地区：[1]解放军总医院第一附属医院检验科,北京100048

出　　处：《感染．炎症．修复》2013年第2期107-109,共3页Infection Inflammation Repair

摘　　要：目的:采用化学发光技术检测乙肝前S1蛋白(pre-S1)和乙肝两对半,探讨乙肝pre-S1作为反映乙肝病毒感染、复制、传染性及肝细胞损伤指标的临床意义。方法:采用化学发光技术检测337例乙肝表面抗原阳性患者的血清乙肝两对半和pre-S1,比较不同乙肝两对半模式患者pre-S1的阳性率。结果:乙肝表面抗原的S/CO值高低与pre-S1有关,乙肝表面抗原S/CO值越高,pre-S1的阳性率越高,不同两对半模式pre-S1阳性率不同,其中以"大三阳"[HBsAg(+)/HBeAg(+)/HBcAb(+)]的阳性率最高(93.3%)。乙肝e抗原与pre-S1结果差异有显著性(P<0.01)。结论:化学发光技术检测前S蛋白能较早以及较准确地反映乙肝病毒复制的情况,对于条件不适合做乙肝病毒DNA的实验室,pre-S1是乙肝两对半的补充。Objective：To explore the clinical value of determination of hepatitis B virus （HBV） pre-S1 in detection of HBV replication, infectivity, and liver cell injury with chemiluminescent assay. Methods： Chemilumines- cent assay was used to determine serum levels of pre-S1 protein and HBV-M （HBsAg, HBsAb, HBeAg, HBeAb, HBcAb） in 337 HBsAg positive cases, then the levels of pre-S1 protein in different HBV-M groups were com- pared. Results.. S/CO value of HBsAg was related to the level of pre-S1 protein, as it was increased with the in- crease of the pre-S1 protein level. The positive rate of pre^S1 was different in different modality of two pair semi- hepatitis B, and it was highest （93.3%） in patients with HBsAg（＋）/HBeAg（＋）/HBcAh（＋）. There was a significant difference between HBeAg and HBV pre-S1 protein（P^0.01）. Conclusions： HBV pre-S1 protein detected with chemiluminescent assay could reflect the replication of HBV earlier and more correctly. Therefore, in the lab where HBV-DNA detection is not available, HBV pre-S1 protein is a complement for HBV-M.

关 键 词：乙肝前S1蛋白 化学发光技术 乙型肝炎病毒 

分 类 号：R512.620.1[医药卫生—内科学]