莱菔硫烷对高糖刺激下大鼠晶状体上皮细胞的抗氧化效应研究  

作　　者：吴恺[1] 谭钢[1] 邵毅[2] 刘二华[1] 

机构地区：[1]南华大学附属第一医院眼科,湖南421001 [2]南昌大学第一附属医院眼科,江西330006

出　　处：《当代医学》2013年第24期1-2,共2页Contemporary Medicine

基　　金：国家自然科学基金(81100648;81160118)

摘　　要：目的研究莱菔硫烷对高糖刺激下大鼠晶状体上皮细胞血红素氧合酶1(hemeoxygenase-1,HO-1)和核因子相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)表达的影响。方法人LECs系SRA01/04细胞用含葡萄糖5.5mmol/(L正常对照组)和30.5mmol/(L高糖组)的培养液中培养。观察组用不同浓度SFN处理24h后,Western blot观察HO-1的表达以及Nrf2的核转位。结果正常对照组SRA01/04细胞中HO-1表达量非常低,高糖处理后,HO-1表达水平有所增加。但SFN处理后,HO-1的表达随着SFN浓度的增高而进一步增高;SRA01/04细胞在正常对照组中Nrf2主要位于细胞浆内,细胞核中Nrf2含量极低。高糖组细胞核中Nrf2仅轻度增加。而SFN处理后,细胞核内Nrf2表达量显著增高。此外,SFN组经Nrf2 siRNA干扰后,HO-1的表达量显著降低。结论 SFN可通过激活Nrf2诱导大鼠晶状体上皮细胞表达HO-1,从而发挥抗氧化作用。Objective To elucidate the antioxidant effect of Sulforaphane （SFN） in on the expression of hemeoxygenase 1 （HO-l） and nuclear factor erythroid 2-related factor 2 （Nrf2） in rat lens epithelial cells cultured in vitro with high glucose.Methods Human lens epithelial cell line SRA 01/04 was cultured in vitro containing 5.5 mmol/L or 30.5 mmol/L glucose as control and high-glucose group, repectively. The observation group was treated by different concentration of SFN for 24 h, expression of heme oxygenase 1 （HO-1） and nuclear translocation were detected by Western blot. Results Expression of HO-I in the control group was very low, and high-glucose could slightly increase the HO-1 level. SFN treatment could increase the expression of HO-1 in dose-dependent manner. Nrf2 was in the cytoplasm, and was very low in nucleolus, high-glucose could only increase Nrf2 nuclear translocation by mild. However, SFN could significantly promote Nrf2 translocate to the nucleus, In addition, silencing of Nrf2 could profoundly inhibit SFN-induced HO-1 expression. Conclusion SFN could induce rat lens epithelial cells expression of HO-1, and this mechanism may be involved in beneficial effect against high glucose- induced oxidative stress.

关 键 词：莱菔硫烷 血红素氧合酶1 核因子相关因子2 晶状体上皮细胞 

分 类 号：R587.2[医药卫生—内分泌] R776.1[医药卫生—内科学]