Lactadherin促进脐带血树突状细胞刺激的T淋巴细胞增殖  

Lactadherin promoting the proliferation of T lymphocyte stimulated by dendritic cell in cord blood

作　　者：李劲松 张利 段宝珍 谢小军 张玉清 江星 时会菊 

机构地区：[1]广东省深圳市南山区西丽人民医院儿科,518055

出　　处：《蚌埠医学院学报》2013年第8期935-938,共4页Journal of Bengbu Medical College

基　　金：广东省深圳市科技计划资助项目(201003294)

摘　　要：目的:探讨lactadherin对树突状细胞(DCs)刺激T淋巴细胞增殖及细胞因子分泌功能的影响。方法:采用密度梯度离心法分离脐带血中单个核细胞并诱导分化为成熟DCs,lactadherin实验组在分离第1天或第5天加入培养液,成熟DCs与异体T淋巴混合培养,通过MTT及CFSE法检测T淋巴细胞增殖水平,ELISA方法检测培养上清中细胞因子的水平。结果:Lactadherin能够显著提高成熟DCs刺激T淋巴细胞的增殖能力,在DCs与T细胞不同比例共培养中均是如此(P<0.01),第5天加入组中现象更加明显(P<0.01)。同时lactadherin作用的DCs可提高T细胞分泌多种细胞因子的水平,尤以第0天加入组中γ-干扰素的分泌增高最为显著(P<0.05)。结论:Lactadherin能够增强DCs刺激T淋巴细胞增殖的能力,从而激活适应性免疫应答。Objective： To explore the effects of lactadherin on promoting the proliferation of T lymphocyte stimulated by dendritic cells（DCs） and cytokines secretion.Methods： Cord blood monocyte was isolated by density gradient centrifugation,and induced and differentiated into DCs.Culture solution was added into the lactadherin experimental group at day 1 and day 5 after isolation.The mixed culture of mature DCs and allogeneic T lymphocytes were implemented.T lymphocyte proliferation were detected using MTT and CFSE,the levels of cytokines were measured using ELISA.Results： Lactadherin could obviously promote the proliferation of T lymphocyte stimulated by DCs（P 0.01）,the result of coculture of DCs and different proportion T lymphocyte was similar,and the ability of T lymphocyte proliferation was more significant at day 5 after adding lactadherin（P 0.01）.DCs induced by lactadherin could promote the level of T cell secreting a variety of cytokines,the level of interferon-γ increased significantly at the time of adding lactadherin（P 0.05）.Conclusions： lactadherin can promote the proliferation of T lymphocyte stimulated by DCs to activate adaptive immune response.

关 键 词：树突状细胞 乳凝集素 T淋巴细胞 增殖 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]