显微注射转基因用猪胚胎获得研究  

Research on Preparation Pig Embryos for Gene Transfer by Microinjection

作　　者：李文化[1] 樊俊华[2] 郑新民[2] 魏庆信[2] 沈萍[1] 

机构地区：[1]武汉大学生命科学院,湖北武汉430072 [2]湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《中国畜牧杂志》2000年第6期5-6,共2页Chinese Journal of Animal Science

摘　　要：本文从猪胚胎的体外成熟和体内成熟两方面对显微注射用猪胚胎的获取进行了初步研究。实验结果表明 ;超数排卵母猪的手术冲胚是获取显微注射用猪胚胎的最佳方法 ,该方法获得的胚胎数量多 ,质量好 ,转基因效率高。体外成熟法获得可供显微注射用猪胚胎的效率低 ,基因转移的总效率也很低。Maturation in vitro and in vivo are two ways to prepared pig embryos for microinjection.Maturation in vitro includes oocyte maturation culture and fertilization;and maturation in vivo is a method of getting embryos directly from natural and superovulation sows. Experiment results indicated that obtaining embryos from superovulation sows by operation is the best way to get more and better embryos, and the gene transfer efficiency is higher. On the contrary, the efficiency of preparation embryos for microinjection by maturation in vitro is low, and the efficiency of gene transfer is low too.

关 键 词：显微注射 猪胚胎 体外成熟 体内成熟 转基因技术 

分 类 号：S814.8[农业科学—畜牧学] S828.3[农业科学—畜牧兽医]