大肠杆菌核心型血清学检测方法的建立  

The establishment of serologically detecting method on core type of Escherichia coli

作　　者：柯岩[1] 周燕竹[2] 沈海中[1] 黄恕[3] 陈海伦[1] 安云庆[1] 

机构地区：[1]首都医科大学微生物学和免疫学教研室 [2]北京同仁医院 [3]北京针灸骨伤学院微生物学教研室

出　　处：《中华微生物学和免疫学杂志》2000年第6期594-597,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　制备大肠杆菌 (E .coli)核心型单一特异性抗血清 ,建立E .coli核心型血清学检测法 ,检测致泻性E .coli核心型。方法　ELISA间接法 ,用吸收后单一核心型特异性抗血清检测鉴定E .coli核心型。结果　 (1)吸收后获得单一核心型特异性抗血清 ,对E .coli核心型的检测结果与相应单克隆抗体检测结果完全相同 ;(2 )经鉴定表明 ,吸收后单一核心型特异性抗血清识别结合的抗原成分既非E .coli核酸和蛋白 ,又非E .coli类脂A和内核心KDO 庚糖 ,而是构成E .coli核心型的外核心己糖物质 ;(3)四类 2 7株不同血清型致泻性E .coli核心型检测结果为 :R1核心型 15株 (5 5 .5 % )、R3核心型 10株 (37.1% ) ,K12核心型 2株 (7.4% ) ,未检测出R2和R4核心型。结论　吸收后E .coli核心型抗血清具有较高单一核心型特异性 ,可用于E .coli核心型的检测。Objective To prepare the core type monospecific antisera of Escherichia coli (E.coli). To establish serological detecting method for core types of E.coli. To detect the distribution of lipopolysacchride core types in pathogenic E.coli. Methods ELISA indirect method, we detected lipopolysacchride core types of E.coli using absorbed lipopolysacchride core type monospecific antisera in E.coli. Results (1) The respective core type antisera were made as a monospecific one by repeated absorption. Lipopolysacchride core types of E.coli by absorbed lipopolysacchride core type monospecific antisera is same as that by the monoclone antibody; (2) The results proved that the components being recognized and bound by absorbed core type monospecific antisera was neither nuclei acid and protein nor lipopolysaccharide lipid A and inner core KDO heptopyranose, an but outer core hexose antigen composing E.coli lipopolysaccharide core types; (3) Lipopolysaccharide core types of 27 strains of different serotype pathogenic E.coli from 4 classes had been detected. The results showed: 15 strains (55.5%) expressed R1 core type, and 10 (37.1%) and 2 (7.4%) strains expressed R3 and K12 core types respectively. None of the 27 strains of E.coli expressed R2 and R4 core type. Conclusion The respective core type antisera were made higher monospecific by repeated absorption, and it can be used in detecting E.coli lipopolysaccharide core types.

关 键 词：致泻性大肠杆菌 核心型异性抗血清 ELISA 

分 类 号：R446.61[医药卫生—诊断学]