不同长度疟原虫环子孢子蛋白重复序列DNA免疫的实验分析  

A Preliminary Analysis on the DNA Vaccination with Different Sizes of Repeat Sequences of Circumsporozoite Protein Gene

作　　者：孙明[1] 王炯[1] 董承红[1] 王丽春[1] 李琦涵[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,昆明650118

出　　处：《上海免疫学杂志》2000年第6期326-328,共3页Shanghai Journal of Immunology

摘　　要：本实验利用DNA免疫技术 ,将PCR得到的疟原虫环子孢子蛋白 (CSP )不同长度的重复序列基因克隆到pcDNA3质粒中 ,对小鼠进行肌肉注射 ,经过免疫检测 ,结果证明不同长度CSP的重复序列能够单独诱发机体全面的细胞免疫和体液免疫 ,这为今后疟疾疫苗的发展提供了新的思路。The different sizes of repeat sequences encoding the CSP of Plasmodium falciparium were cloned into plasmid pcDNA3 and BALB/c mice were inoculated intramuscularly with this recombinant plasmid DNA It was found that the vaccination with this plasmid DNA could elicite both humoral and cellular immune responses These results privide a new way of approaches to the reseach of human malaria vaccines

关 键 词：DNA免疫 GSP基因重复序列 疟疾 疫苗 CTL 

分 类 号：R392－33[医药卫生—免疫学] R531.303[医药卫生—基础医学]