羟基磷灰石纳米微粒对不同种类低温保护剂反玻璃化结晶的影响  

Effects of Hydroxyapatite Nanoparticles on Devitrification Crystallization of Different Kinds of Cryoprotectants

作　　者：吕福扣[1] 刘宝林[1] 李维杰[1] 

机构地区：[1]上海理工大学生物热科学研究所,上海200093

出　　处：《制冷学报》2013年第4期18-23,共6页Journal of Refrigeration

基　　金：国家自然科学基金(51076108);教育部新世纪优秀人才计划(07-0559)资助项目;上海市东方学者计划项目资助~~

摘　　要：为了研究羟基磷灰石(HA)纳米微粒对不同种类低温保护剂(质量浓度为60%的渗透性丙三醇和质量浓度为50%的非渗透性PEG-600)反玻璃化结晶的影响,利用低温显微镜研究了含有不同粒径(20nm、40nm、60nm)和不同质量浓度(0.1%、0.5%)HA纳米微粒的丙三醇和PEG-600溶液在反玻璃化过程中的结晶现象。实验结果表明:丙三醇和PEG-600溶液在反玻璃化过程中的冰晶形貌分别为枝状和球形,且形貌都不随添加纳米微粒而改变。与未添加纳米微粒的丙三醇溶液相比,添加60nm、0.5%纳米微粒溶液的析晶分数仅为未添加溶液的1/7。PEG-600溶液在-64℃^-52℃区间内的析晶分数显著增加,其中添加40nm、0.5%纳米微粒的PEG-600溶液的析晶分数增加了90%。冰晶的生长速率存在两个极大值现象,而添加40nm、0.5%纳米微粒的丙三醇溶液、未添加和添加60nm纳米微粒的PEG-600溶液的冰晶生长速率都只有一个极大值。To study the effects of Hydroxyapatite （HA） nanoparticles on devitrification of different cryoprotectant solutions, the crystalli- zation of Glycerol（ mass concentration is 60% ） and PEG-600（ mass concentration is 50% ） solutions with HA nanoparticles of different particle size（20nm,40nm ,60nm）and concentrations（0.1% ,0.5% ）were observed under a cryomicroscope. Experimental resuhs showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanopartieles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between - 64℃ and - 52℃, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nrn, and PEG-600 solution without nanoparticles.

关 键 词：低温显微镜 反玻璃化 冰晶形貌 冰晶分数 冰晶生长速率 

分 类 号：TB383[一般工业技术—材料科学与工程] TQ171.1[化学工程—玻璃工业]