银杏单细胞克隆研究  

Studies on Single Cell Cloning of Ginkgo biloba L.

作　　者：戴均贵[1] 朱蔚华[1] 吴蕴祺 胡秋[1] 张大勇 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《中国中药杂志》2000年第10期593-597,共5页China Journal of Chinese Materia Medica

摘　　要：目的 :利用银杏单细胞克隆系 ,探索银杏内酯生产的稳定性与细胞均一性之间的关系。方法 :利用植物细胞平板技术培养。结果 :L 谷氨酰胺能大大促进银杏细胞的植板率 ;植板率在 0 .5～ 5 .0× 10 4 cell·ml-1随密度的增加而升高。获得的 48个克隆株系中G 2 2株系银杏内酯B(GKB)含量高达 0 .0 99% ,且大部分克隆株系在继代培养过程中能保持相对稳定。结论 :在银杏组织培养过程中 ,利用单细胞克隆技术可在一定程度上解决银杏内酯生产不稳定性的问题。Objective:To investigate the relations of the productivity stability of to the homogenization of Ginkgo biloba cells by single cell clones.Method:Using plant single cell plate culture technique.Results:Adding 500 ml·L -1 L glutamine in the medium increased greatly the cell plating efficiency (PE),and within the range 0.5～5.0×10 4 Cell·ml -1 of the initial cell density,the PE increased with the increment of the cell density.Of the 48 cell clones obtained,the GKB content in G 22 clone reached 0.099%;and most of the clones could remain stable in growth and GKB production during subcultures.Conclusion:During the cell and tissue culture of G. biloba,the application of plant single cell cloning technique helps to a certain extent solve the problem of productivity instability of ginkgolide. [

关 键 词：银杏 单细胞克隆 银杏内酯B 

分 类 号：R282[医药卫生—中药学]