桂枝汤有效部位A对下丘脑热休克蛋白含量的影响  

Effect and Mechanism of Active Fraction A Guizhi Decoction on Dual-Directional Thermoregulation: Effect on Heat Shock Protein in Hypothalamus of Rats

作　　者：霍海如[1] 谭余庆[1] 周爱香 李晓芹[1] 郭淑英[1] 孙玉茹[1] 姜廷良[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2000年第10期619-621,共3页China Journal of Chinese Materia Medica

基　　金："九五"攀登计划预选项目

摘　　要：目的 :研究桂枝汤有效部位A(Fr.A)在双向调节体温的同时对下丘脑热休克蛋白 (HSP)的影响。方法 :取高、低体温大鼠下丘脑组织 ,应用蛋白印迹法测定热休克蛋白含量。结果 :Fr.A在双向调节体温的同时 ,可明显拮抗安痛定性低体温大鼠下丘脑HSP70含量的降低 ,对酵母性高体温大鼠下丘脑HSP70含量具有一定的降低作用。结论 :Fr.A对高、低体温大鼠下丘脑HSP70含量的调节作用是其双向调节体温的机理之一。Objective: To investigate the effect of active fraction A in Guizhi Decoction (Fr.A) on dual directional thermoregulation and its mechanism of influencing heat shock protein (HSP) in hypothalamus. Method:Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats. Results:Regulating the body temperature in dual direction,Fr.A could antagonize the decrease of HSP contents of hypothalamus in hypothermal rats induced by aminopyrine, and abate the HSP content in febrile rats induced by yeast. Conclusion: Fr.A adjusts the body temperature through regulating the contents of HSP of hypothalamus in febrile and hypothermal rats. [

关 键 词：桂枝汤 下丘脑 热休克蛋白 蛋白印迹法 

分 类 号：R285.5[医药卫生—中药学]