三磷酸腺苷法肿瘤药敏实验在筛选鼻咽癌新辅助化疗药物中的应用  

Application of ATP Assay in Vitro Bioluminescence Tumor Sensitivity to Neoadjuvant Chemotherapy for Nasopharyngeal Carcinoma

作　　者：何志坚[1] 钟睿[1] 刘海云[2] 敖帆[1] 

机构地区：[1]江西省肿瘤医院,江西南昌330029 [2]江西中医药大学基础医学院,江西南昌330004

出　　处：《肿瘤药学》2013年第4期259-263,共5页Anti-Tumor Pharmacy

基　　金：江西省卫生厅科技项目(No.20063177);江西省卫生厅中医药科研基金项目(No.2011A137);江西省教育厅青年科学基金项目(No.GJJ12541)

摘　　要：目的探讨肿瘤体外药敏实验ATP生物荧光法(ATP-TCA)在筛选鼻咽癌新辅助化疗药物中的应用。方法应用ATP-TCA法体外检测84例鼻咽癌活检组织对8种常用化疗药物的敏感性。结果组织标本的可评估率为100.0%。PXT最敏感,体外有效率为50.00%,其次为DDP(35.45%)、5-Fu(31.25%)、NVB(23.53%)、HCT(10.53%)、MTX(10.53%)、VP-16(7.69%)、THP(5.00%)。化疗药物对鼻咽癌的杀伤作用具有较强的个体差异性。结论体外鼻咽癌组织对8种化疗药物敏感性不同,存在明显差异性,ATP生物荧光肿瘤体外药物敏感性检测法可用于临床制定个体化的鼻咽癌新辅助化疗方案。Objective To investigate the drug sensitivity of nasopharyngeal carcinoma in vitro by ATP-TCA, and provide basis ofneoadjuvant chemotherrap in nasopharyngeal carcinoma. Methods ATP-TCA was applied to determine the drug sensitivity of 84 nasopharyngeal carcinomas to eight common chemotherapeutics. Results The rate of evaluation of tissue samples was 100%. The drug with most sensitive efficiency was PXT （50%）, followed by DDP （35.45%）, 5-Fu （31.25%）, NVB （23.53%）, HCT （10.53%0, MTX （10.53%）, VP-16 （7.69%）, THP （5.00%,）. The chemotherapy drugs showed strong individual difference in the killing effect on nasopharyngeal carcinoma. Conclusions The nasopharyngeal carcinoma tissues in vitro had significantly different sensitivity to eight kinds of chemotherapy drugs. ATP bioluminescence tumor drug sensitivity assay can be used in the clinical setting of individualized neoadjuvant chemotherapy for nasopharyngeal carcinoma （NPC） patients.

关 键 词：药物敏感性试验 ATP-生物荧光法 鼻咽癌 新辅助化疗 

分 类 号：K73-34[历史地理—历史学] R739.6[历史地理—世界史]