HPLC与LC-MS/MS测定蛹虫草发酵液中虫草素的方法比较  

Determination of Cordycepin in Fermentation Supernatant of Cordyceps militaris by HPLC and LC-MS/MS: A Comparison of Two Methods

作　　者：简利茹[1,2] 杜双田[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]旱区作物逆境生物学国家重点实验室(西北农林科技大学),陕西杨凌712100

出　　处：《食品科学》2013年第14期276-279,共4页Food Science

基　　金：陕西省科技统筹创新工程计划项目(2011KTCL02-16)

摘　　要：目的:通过高效液相色谱(HPLC)与液质联用(LC-MS/MS)测定蛹虫草发酵液中虫草素的方法比对,评价2种方法的可行性。方法:蛹虫草发酵液经大孔树脂分离纯化后,分别采用紫外检测器和质谱检测器对10种不同发酵液进行测定。结果:HPLC法测定发酵液中虫草素的检出限(LOD)和定量限(LOQ)分别为13.6μg/L和53.6μg/L,LC-MS/MS法测得虫草素的LOD和LOQ分别为2.1μg/L和7.8μg/L,2种方法虫草素的保留时间分别为22.7min和7.8min,平均回收率分别为94.68%和93.91%。结论:两种方法灵敏度高、选择性好、操作简便,均可对蛹虫草发酵液中虫草素进行准确的定性定量分析。Objective： To comparatively evaluate the feasibility of HPLC and LC-MS/MS for measuring cordycepin in fermentation supernatant of Cordyceps militaris.Methods： The fermentation supernatants of 10 Cordyceps militaris strains were purified by macroporous resin column chromatography.The purified samples obtained were analyzed separately by HPLC with UV detection and LC-MS/MS.Results： The limit of detection（LOD） and limit of quantitation（LOQ） of HPLC were 13.6 μg/L and 53.6 μg/L,respectively,whereas those of LC-MS/MS were 2.1 μg/L and 7.8 μg/L,respectively.The retention time of cordycepin in HPLC and LC-MS/MS was 22.7 min and 7.8 min,respectively,and the average recovery was 94.68% and 93.91%,respectively.Conclusion： Both methods have high sensitivity,selectivity and simple operation,and can be used for qualitative and quantitative analysis.

关 键 词：高效液相色谱 液质联用 蛹虫草发酵液 虫草素 

分 类 号：O657.72[理学—分析化学]