UPLC同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚、和厚朴酚的含量  

Simultaneous determination of six ingredients in Huoxiang Zhengqi oral liquid by UPLC

作　　者：余佳文[1] 邓开英 彭涛[1] 朱必越[4] 刘红亚[5] 

机构地区：[1]太极集团重庆涪陵制药厂有限公司,重庆408000 [2]重庆市食品药品检验所,重庆401121 [3]重庆市药物过程与质量控制工程技术研究中心,重庆401121 [4]四川大学华西药学院,四川成都610041 [5]四川省中医药科学院,四川成都610041

出　　处：《中国中药杂志》2013年第14期2314-2317,共4页China Journal of Chinese Materia Medica

摘　　要：目的:建立同时测定藿香正气口服液中甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、厚朴酚以及和厚朴酚6个成分的UPLC分析方法。方法:采用Zorbax Eclipse C18柱,以乙腈(A)-0.05%磷酸溶液(B)为流动相,梯度洗脱(0～5 min,17%～20%A,5～12 min,20%～50%A,12～18 min,50%～70%A,18～18.5 min,70%～95%A,18.5～23.5 min,95%A),流速0.42mL.min-1,检测波长220 nm,柱温30℃。结果:甘草苷、柚皮芸香苷、橙皮苷、甘草酸铵、和厚朴酚以及厚朴酚进样量分别在0.001 7～0.034,0.003 4～0.068,0.006 4～0.128,0.012 8～0.256,0.003 2～0.064,0.006 4～0.128μg线性关系良好,平均加样回收率分别为103.3%,98.39%,98.29%,102.1%,98.45%,102.2%,RSD分别为2.1%,1.0%,1.5%,2.3%,0.90%,2.0%。结论:UPLC分离效果及重复性好,且快速简便,可作为藿香正气口服液的质量控制方法。Objective： To develop a UPLC method for the simultaneous determination of liquiritin,narirutin,hesperidin,ammonium glycyrrhetate,honokiol and magnolol in Huoxiang Zhengqi oral liquid.Method： A Zorbax Eclipse C18 column was used with the mobile phase of acetonitrile and 0.05% phosphate acid by gradient elution at the detection wavelength of 220 nm.The flow rate was 0.42 mL· min-1 and the column temperature was 30 ℃.Result： The calibration curves were linear in the ranges of 0.001 7-0.034,0.003 4-0.068,0.006 4-0.128,0.012 8-0.256,0.003 2-0.064,0.006 4-0.128 μg,respectively.The average recoveries were 103.3%,98.39%,98.29%,102.1%,98.45%,102.2% with RSDs of 2.1%,1.0%,0.50%,2.3%,0.9%,2.0%,respectively.Conclusion： The UPLC method was simple,rapid and accurate,it could be used for quality control of Huoxiang Zhengqi oral liquid.

关 键 词：藿香正气口服液 甘草苷 柚皮芸香苷 橙皮苷 甘草酸铵 和厚朴酚 厚朴酚 UPLC 

分 类 号：R286[医药卫生—中药学]