柔毛鸦胆子茎的化学成分及其肿瘤细胞毒活性研究  

Chemical constituents from stems of Brucea mollis and their cytotoxic activity

作　　者：陈辉[1,2] 柏健[1] 方振峰[1] 马双刚[1] 庾石山[1] 陈晓光[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050 [2]河南中医学院药学院,河南郑州450046

出　　处：《中国中药杂志》2013年第14期2321-2324,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2012ZX09301002-002)

摘　　要：运用各种柱色谱方法和制备型HPLC技术对柔毛鸦胆子茎的化学成分进行分离纯化,共从其茎的95%乙醇提取物中分离得到10个化合物,采用NMR等波谱技术鉴定其结构分别为deacetylated isobrucein B(1),indaquassin X(2),cleomiscosin A(3),cleomiscosin B(4),(+)-lyoniresinol(5),(+)-表松脂醇(6),(+)-松脂醇(7),(+)-丁香脂素(8),4,5-dihydroblumenol A(9),腺苷(10)。采用MTT法对以上化合物进行了体外细胞毒活性测试,化合物2对人源肿瘤细胞HT-29,HepG2,BGC-823和SKOV3具有显著的细胞毒活性(IC500.84～3.97μmol.L-1)。所有化合物均为首次从该植物中分离得到,其中化合物1是1个新天然产物。化合物2对人源肿瘤细胞HT-29,HepG2,BGC-823和SK-OV3具有显著的细胞毒活性。Ten compounds were isolated from the stems of Brucea mollis by various chromatographic techniques such as column chromatography on silica gel and Sephadex LH-20,and preparative HPLC,and their structures were elucidated as deacetylated isobrucein B（1）,indaquassin X（2）,cleomiscosin A（3）,cleomiscosin B（4）,（＋）-lyoniresinol（5）,（＋）-epipinoresinol（6）,（＋）-pinoresinol（7）,（＋）-syringaresinol（8）,4,5-dihydroblumenol A（9） and adenosine（10） on the basis of spectroscopic data analysiS.All compounds were obtained from this plant for the first time,moreover,compound 1 was a new natural product.Compound 2 showed significant cytotoxic activities against the human cell lines HT-29,HepG2,BGC-823 and SKOV3 with IC50 values of 0.84-3.97 μmol·L-1.

关 键 词：苦木科 柔毛鸦胆子 化学成分 细胞毒活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]