当归多糖对小鼠衰老造血干细胞端粒、端粒酶及P53的影响  

Effect of Angelica sinensis polysaccharide on expression of telomere,telomerase and P53 in mice aging hematopoietic stem cells

作　　者：张先平[1] 刘俊[1] 徐春燕[1] 魏强[1] 李静[1] 王璐[1] 王建伟[1] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室组织胚胎学教研室,重庆400016

出　　处：《中国中药杂志》2013年第14期2354-2358,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173398;30970872)

摘　　要：目的:观察当归多糖(ASP)对小鼠造血干细胞(HSC)端粒长度、端粒酶活性及P53表达的影响,探讨ASP调控HSC衰老的可能机制。方法:C57BL/6J小鼠随机分为正常组、衰老组和干预组,衰老组采用X线全身均匀照射,建立小鼠HSC衰老模型;干预组在照射期间给予ASP灌胃;正常组给予NS灌胃。免疫磁珠分离HSC,运用细胞周期分析和β-半乳糖苷酶(SA-β-Gal)染色观察HSC衰老生物学变化;Western blot检测P53蛋白表达,Southern blot和TRAP-PCR分别检测HSC端粒长度和端粒酶活性。结果:与正常组比较,X线能显著增加衰老组HSC G1期细胞比例、SA-β-Gal染色阳性细胞率及P53蛋白表达;降低端粒长度和端粒酶活性。与衰老组比较,ASP能显著抑制衰老HSC G1期细胞比例及SA-β-Gal染色阳性细胞率的增加;下调P53蛋白表达;增加端粒长度和端粒酶活性。结论:ASP能够拮抗X线诱导的HSC衰老,其作用机制可能与增加端粒长度及端粒酶活性、下调P53蛋白表达有关。Objective： To observe the effect of Angelica sinensis polysaccharides（ASP） on the length of telomere,the activity of telomerase and the expression of P53 protein in mice hematopoietic stem cells（HSCs）,and explore ASP′s potential mechanism for regulating HSC aging.Method： C57BL/6J mice were randomly divided into the normal group,the aging group and the intervention group.The aging group was radiated with X ray to establish the mice aging HSC model.The intervention group was orally administered with ASP during X-ray irradiation,while the normal group was orally administered with NS.Their HSCs were isolated by immunomagnetic beads.Cell cycles analysis and senescence-associated β-galactosidase（SA-β-Gal） staining were used to detect changes in aging HSCs.The expression of P53 was determined by western blot analysis.The length of telomere and the vitality of telomerase were analyzed by southern blot and TRAP-PCR,respectively.Result： Compared with the normal group,X-ray irradiation could significantly increase the cell ratio of in HSC G1 stage,rate of SA-β-Gal positive cells and expression of P53 protein,and reduce the length of telomere and the vitality of telomerase.Compared with the aging group,ASP could significantly inhibit the cell ratio of in HSC G1 stage and the increase in the number of SA-β-Gal positive cells,down-regulate the expression of P53 protein,and increase the length of telomere and the vitality of telomerase in HSCs.Conclusion： ASP could antagonize X-ray-induced aging of HSCs,which may be related to the increase in the length of telomere and the activity of telomerase,as well as the down-regulation of the expression of P53 protein.

关 键 词：当归多糖 造血干细胞 细胞衰老 端粒 

分 类 号：R285[医药卫生—中药学]