大黄属药用植物抗HIV-1活性的谱效关系研究  

Spectrum-effect relationship between anti-HIV 1 activities and ultraperformance liquid chromatography fingerprints of Rheum species

作　　者：马培[1] 张鑫瑶[1] 许利嘉[1] 王哲[1] 肖培根[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,北京100193

出　　处：《中国中药杂志》2013年第15期2434-2437,共4页China Journal of Chinese Materia Medica

基　　金：国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10005-004)

摘　　要：目的:分析大黄属药用植物的超高效液相色谱法(UPLC)特征图谱与抗HIV-1活性之间的相互关系。方法:采用HIV-1逆转录酶相关的核糖核酸酶H活性测定检测了大黄属16种22个样品的抗HIV-1活性;同时测定了22个样品的UPLC特征图谱;运用数理统计方法研究谱效相关性;并采用UPLC-TOF-MS/MS指认样品主要成分峰。结果:藏边大黄、苞叶大黄和河套大黄提取物有较好的抗HIV-1活性。谱效相关性研究表明4.74,7.99,21.18 min色谱峰与活性有一定相关性。结论:通过谱效研究,推测了有抗HIV-1活性的大黄属药用植物及其活性组分,为大黄属植物深层次的研究开发奠定了基础。Objective： To study the relationship between ultra-performance liquid chromatography （UPLC） fingerprints of Rheum species and their anti-HIV 1 activities. Method： Twenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase （RT） associated ribonuclease H （RNase H） activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method. Result： Samples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50≤0.2 mg·L-1. The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively. Conclusion： Compounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.keywords：Rheum species

关 键 词：大黄 特征图谱 抗HIV-1活性 谱效关系 

分 类 号：R285.5[医药卫生—中药学]