艾滋病免疫重建不全患者TCRVβ基因多样性改变及药物干预研究  

Diversity changes of TCRVβ gene in AIDS patients with incomplete immune reconstitution and influence of drug

作　　者：汤艳莉 王阶[2] 李勇[2] 刘咏梅[2] 

机构地区：[1]泰达医院,天津300457 [2]中国中医科学院广安门医院,北京100053

出　　处：《中国中药杂志》2013年第15期2438-2442,共5页China Journal of Chinese Materia Medica

基　　金：国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10005-004)

摘　　要：目的:探讨艾滋病免疫重建不全患者的TCRVβ基因多样性改变及中药免疫2号方的干预作用。方法:采集37例艾滋病免疫重建不全患者治疗前后的外周血PBMC,另以15例HIV抗体阴性健康献血员做对照,采用人类TCRVβ基因CDR3多样性定量检测试剂盒检测,基因扫描作图并计算Vβ家族中每个家族不同大小CDR3区片段的分布。结果:HAART治疗后1年免疫重建不全患者的TCRVβ多个家族的扫描图高斯分布被打破,并发生TCR谱系的偏移,经半年中药联合HAART治疗后,偏移的TCR谱系有所恢复。以试剂盒提供的定量分析软件计算的距离D(distance)值来量化评价,2组D值改变无明显差异,但联合中药能减少数据的变异性。CD4+T细胞计数与TCRVβ基因多样性改变两者存在显著的相关关系(r=-0.772,P=0.000)。结论:中药复方对于TCRVβ各家族单寡克隆情况有不同程度的改善和恢复,提示中医药可能促进T细胞部分受体基因重排,丰富受体库,帮助机体免疫细胞有效识别病毒,减少T细胞凋亡。Objective： To discuss the drug intervention in diversity changes of TCRVβ gene in AIDS patients with incomplete immune reconstitution. Method： PBMCs were isolated from 37 cases of AIDS patients failure to immune reconstitution before and after treatment with Immune 2 and 15 cases of HIV negative healthy donors. The human gene TCRVβ CDR3 diversity quantitative detection reagent box were used, and mapped the distribution of gene scanning and calculated different CDR3 fragme of each Vβ family size. Result： ①Gaussian distribution of TCRVβ families in patients with incomplete immune reconstitution after one year of HAART, had been broken with the occurrence of the offset TCR lineage. After six months of treatment of traditional Chinese medicine combined HAART, the TCR lineage has been partially restored. ②Evaluated by the D （distance） value calculated by a quantitative analysis software which the kit provides, there were no significant difference in D value change between the two groups, but with traditional Chinese medicine can reduce the data variability.③CD4＋T cell counts had a significant correlation （r=-0.772, P=0.000）with TCRVβ genetic diversity. Conclusion： Study of the mechanism showed oligoclonal of TCRVβ family can get recovery in some degrees after treated by Immune 2 plus HAART, suggesting that the medicine may promote T-cell receptor gene rearrangement, helping immune cells to effectively identify the virus to reduce T-cell apoptosis.keywords：AIDS

关 键 词：艾滋病 免疫重建不全 TCRVΒ 免疫2号方 

分 类 号：R512.91[医药卫生—内科学]