细胞红蛋白对新生大鼠缺氧缺血性脑损伤的神经保护作用  

Neuro-protective function of cytoglobin on neonatal rats with hypoxic-ischemic brain damage

作　　者：肖丹萍[1] 杨汉华[1] 史雪川[1] 

机构地区：[1]汕头大学医学院第二附属医院儿科,515000

出　　处：《中华围产医学杂志》2013年第8期493-498,共6页Chinese Journal of Perinatal Medicine

基　　金：广东省医学科研基金（B2009180）

摘　　要：目的探讨细胞红蛋白（cytoglobin，CYGB）在缺氧缺血性脑损伤（ hypoxic-ischemic brain damage, HIBD）中的神经保护作用。方法健康7日龄新生Sprague-Dawlay大鼠随机分为假手术组、HIBD组、HIBD＋氯化高铁血红素（Hemin）组和HIBD＋锌原卟啉（zinc protoporphyrin，ZnPP）组。HIBD组、HIBD＋Heroin组及HIBD＋ZnPP组大鼠分别腹腔注射0．5ml生理盐水、Heroin（50mg／kg）及ZnPP（50mg／kg），12h后结扎并剪断左侧颈总动脉，缺氧2h，制成HIBD动物模型。建立模型后0、24、48h免疫组织化学法检测各组大鼠海马及皮质中CYGB的表达情况，每组10只。HE染色观察建立模型后48h的大鼠脑组织病理变化，每组8只。称重法检测建立模型后72h新生大鼠脑含水量，每组8只。建立模型后28d采用Morris水迷宫实验检测海马学习记忆功能变化，每组10只。组间差异比较采用方差分析，组间两两比较使用Bonferroni法。结果（1）大鼠脑组织CYGB的表达水平：用平均灰度值（与蛋白表达水平成反比）表示。在0h，HIBD＋Hemin组和HIBD组皮质CYGB的平均灰度值分别为166．7±5．1和207．1±5．1，低于似手术组（232．3±3．4），而HIBD＋ZnPP组（234．9±4．5）高于假手术组（P〈0．05）。随时间的增加，各HIBD组CYGB的平均灰度值递减，至48h时，CYGB的平均灰度值HIBD＋Hemin组最低（126．0±2．6），其次是HIBD组（150．9±4．5）和HIBD＋ZnPP组（163．7±6．3），最高是假手术组（232．1±5．8），差异均有统计学意义（P均〈O．01）。CYGB在海马组织的表达变化与皮质一致。（2）各组脑组织病理变化：建立模型后48h，HIBD组、HIBD＋Heroin组和HIBD＋ZnPP组新生大鼠脑组织可见典型的脑梗死和出血，H1BD＋Hemin组梗死灶明显较HIBD＋ZnPP组小，出仇减轻。（3）脑组织含水量变化：建立模型后72h，HIBD组和HIBD4-ZnPP组左侧脑组织含水量分别为（86．5±0．4）％和�Objective To investigate the protective function of cytoglobin （CYGB） on neuro- development of the neonatal rats with hypoxic-ischemic brain damage （HIBD）. Methods Healthyseven-day-old Sprague Dawlay rats were randomly divided into sham operated group, HIBD group, HIBD＋Hemin group and HIBD＋zinc protoporphyrin （ZnPP） group. The rats of HIBD, HIBD＋ Hemin and HIBD＋ZnPP group were given normal saline （0.5 ml）, Hemin （50 mg/kg） and ZnPP （50 mg/kg） intraperitoneally respectively, and 12 hours later the left carotid arteries of these rats were ligated and cut off, then hypoxic treated for 2 hours to establish the HIBD models. At 0, 24, 48 h after HIBI） models were established, the expressions of CYGB in the cerebral cortex and hippocampus were observed by immunohistochemistry analysis. At 48 h after HIBD, histopathological changes of brain were observed after HE staining. At 72 h after HIBD, the water content of the brain was observed. At 28 days after HIBD, long term study-memory outcome was assessed by Morris water maze. Analysis of variance and Bonferroni test were applied as statistical methods. Results （1） The expression of CYGB in brain（expressed by average gray value which negatively correlated with protein levels） ： At 0 h, the average gray value of CYGB in the cerebral cortex in HIBD＋Hemin and HIBD group were 166.7±5. 1 and 207.1±5.1,which were lower than that in sham operated group （232.3±3.4）; but in HIBD＋ZnPP group, it was higher （234.9±4.5） （P〈0.05）. The average gray value of CYGB was decreased with the extension of hypoxic ischemic time. At 48 h, the average gray value of CYGB was the lowest in HIBD＋Heroin group （126.0±2.6） ,followed by H1BD group （150.9±4.5） and HIBD＋ZnPP group （163.7±6.3）,and the highest was in sham operated group （232.1±5.8）（all P〈0.01）. （2） Histopathologic changes of the brain： At 48 h, typical cerebral infarction and hemorrhage were seen in HIBD, HIBD＋ Hemin and HIBD＋ZnPP group, b

关 键 词：缺氧缺血 脑 珠蛋白类 大鼠 

分 类 号：R722.1[医药卫生—儿科]