神经生长因子及其结合肌基膜管移植修复脊髓横断性损伤的组织学观察  

Transplantation of autologous muscle basal lamina and use of nerve growth factor to repair spinal cord transection in dogs: an histological observition

作　　者：李培建[1] 胥少汀[2] 

机构地区：[1]重庆第三军医大学大坪医院野战外科研究所六室,400042 [2]北京军区总医院

出　　处：《中华神经外科杂志》2000年第6期367-370,共4页Chinese Journal of Neurosurgery

摘　　要：目的　对应用神经生长因子 (NGF)及其结合肌基膜管 (MBL)修复脊髓横断性损伤进行组织学评价。方法　横断雌性家犬脊髓后 ,分为三组 :(A)MBL移植结合注射NGF组 7只。 (B)脊髓单纯横断注射NGF组 6只。 (C)对照组切除脊髓 0 5cm 4只。六个月后用免疫组化学方法对神经轴突和胶质细胞网格框架结构 ,进行特异染色 ,并用图像分析方法对脊髓横断处的远近端横截面进行神经纤维数量对比。结果　各组远端神经纤维数量均有显著性差异 ;MBL移植物中有神经纤维通过。对照组断端为坏死空腔。电镜证实横断远端存在新生神经纤维。结论　这些说明MBL移植结合NGF修复脊髓横断性损伤 ,神经轴突能越过移植区到达远端。Objective To observe the histological changes of transplanting autologous muscle basal lamina(MBL) and using nerve growth factor (NGF) to repair spinal cord transection in adult dogs.Methods Laminectomy was done in 17 adult female dogs over T 13 and L 1. The animals were divided into three groups. Group A: 7 dogs, a gap of 1cm was done longitudinally on the spinal cord at the lesion site. Autologous MBL grafts made from paraspinal muscle fibers were implanted to the spinal cord gaps. NGF was administered at the lesion site via indwelling, implaned osmotic minipumps. Group B: 6 dogs. NGF was administered at the lesion site via indwelling, implaned, osmotic minipumps. Purified NGF was supplied. Group C: 4 dogs serve as controls and each animal received a complete transection on the spinal cord. Neurological function was observed for 6 months and the animals were sacrificed. The grafted materials and treated spinal cord were procured for histological examination. The characteristics of regeneration were observed histologically by immuno histocytochemistry methods and electron microscope. All of the immnostaining pictures were analyzed by morphometric analysis which gave out the data of the axons counting.Results The results showed significantly better microscopic and electron microscopic appearance in the group 1 with implantation of both MBL and NGF. Nerve fibers were identified in the graft by immuno histocytochemical staining for NF. Degeneration and necrosis were found in group 3. Little degeneration and necrosis occurred in group 2 and group 1. Conclusions These data showed that MBL can provide a suitable environment for axonal regeneration, and NGF is able to enhance the regeneration activity of central nervous system.

关 键 词：脊髓横断性损伤 神经生长因子 肌基膜管移植 

分 类 号：R651.2[医药卫生—外科学]