川续断种质资源遗传多样性的SRAP分析  

SRAP analysis on genetic diversity for germplasm resources of Dipsacus asper

作　　者：艾强[1] 周涛[1] 江维克[1] 袁媛[2] 肖承鸿[1] 熊厚溪[1] 贺勇 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]中国中医科学院中药资源中心,北京100700 [3]贵州同济堂制药有限公司,贵州贵阳550002

出　　处：《中草药》2013年第15期2155-2159,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学地区基金资助项目(81160501);国家"十一五"科技支撑项目(2009BA174B01);贵阳市科技创新公共技术平台项目(2010筑科合字第3-2号)

摘　　要：目的开展川续断种质资源的遗传多样性研究,为合理利用川续断种质资源提供理论依据。方法运用SRAP分子标记方法对川黔境内川续断的遗传多样性进行分析。结果 10对引物共检测到124个位点,其中102个位点具有多态性,多态位点百分率(PPL)为82.26%。川续断总的Nei’s基因多样性指数(H)为0.280 0,Shannon’s多态性信息指数(I)为0.435 3;居群水平上川续断的PPL为53.92%,H为0.121 2～0.244 0、I为0.179 6～0.361 1,其中5个高海拔、小生境特征的居群遗传多样性指标较高。居群间的基因分化系数Gst为0.293 0,基因流(Nm)为1.206 4。基于遗传相似度,14个居群可聚为3类。结论川续断居群的遗传多样性水平丰富,遗传变异主要存在居群内,地理位置(海拔)和气候是川续断居群遗传多样性较高的影响因素,而地理隔离(小生境)是造成居群内遗传变异高于居群间的另一因素。Objective To study the genetic diversity of Dipsacus asper from different populations and provide a reference for the rational utilization of its germplasm. Methods The genetic diversity of the 14 populations of D. asper was analyzed by SRAP molecular markers. Results Ten pairs of primers produced 124 sites, among which 102 were polymorphic sites. The percentage of polymorphic loci （PPL） was 82.26%. The Nei＇s genetic diversity index （H） and the Shannon＇s information index （I） were 0.280 0 and 0.435 3, respectively. At the population level, PPL was 53.92%, H was 0.121 2-0.244 0, and I was 0.179 6-0.361 1. The genetic diversity values of the five populations were relatively high, and the populations had the characteristics of high altitude and microhabitat. Genetic differentiation coefficient （Gst） was 0.293 0, gene flow （Nm） was 1.206 4. Cluster analysis based on genetic similarity indicated that the 14 populations could be divided into three groups. Conclusion The genetic diversity among the populations ofD. asper was at relatively high level. The genetic variance ofD. asper mainly existed within the populations. The high genetic diversity could be attributed to the geographical position （altitude） and climate, while geographic isolation （microhabitat） was another important factor for the genetic variance within the populations.

关 键 词：川续断 种质资源 遗传多样性 居群 SRAP 

分 类 号：R282.12[医药卫生—中药学]