一测多评法同时测定鱼腥草不同部位中6种活性成分的量  

Simultaneous determination of six active constituent in different parts of Houttuyniae Herba by quantitative analysis of multi-components by single marker

作　　者：何兵[1] 刘艳[1] 李春红[1] 杨世艳[2] 张燕[3] 

机构地区：[1]泸州医学院药物研究中心,四川泸州646000 [2]泸州医学院附属中医医院,四川泸州646000 [3]泸州医学院附属医院,四川泸州646000

出　　处：《中草药》2013年第15期2160-2164,共5页Chinese Traditional and Herbal Drugs

基　　金：泸州市2010年重点科技项目(泸市财企[2010]41号);泸州医学院2010年青年基金(泸医院[2010]108号)

摘　　要：目的建立鱼腥草不同部位中新绿原酸、绿原酸、芦丁、金丝桃苷、异槲皮苷及槲皮苷的一测多评测定方法。方法以Kromasil C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸溶液梯度洗脱,分段变波长测定。以绿原酸为参照物建立与其余成分的相对校正因子,并计算其量,实现一测多评。同时采用外标法测定该6个成分的量,并比较二者的差异,以验证一测多评法的准确性和可行性。结果鱼腥草中6个成分的计算值与实测值间无显著差异,鱼腥草不同部位6个成分量有所不同,绿原酸类和黄酮类主要分布在叶中。结论鱼腥草的药效成分主要集中在叶中,本实验建立的一测多评法可行准确,可以用来对鱼腥草进行质量控制。Objective To establish a method for simultaneous determination of the contents of six main active constituents （neochlorogenic acid, chlorogenic acid, turin, hyperin, isoquercitrin, and quercitrin） in Houttuyniae Herba by quantitative analysis of multi-components by single marker （QAMS）. Methods The chromatographic separation was achieved on a Kromasil C18 column （250 mm × 4.6 ram, 5 μm）, the mobile phase was acetonitrile-0.1% phosphoric acid aqueous with gradient elution and changeable wavelength detection. Chlorogenic acid was used as the reference, the calibration factor of other five constituents to that of chlorogenic acid was calculated respectively to get each relative calibration factor. And the contents of the active constituents were calculated by the relative calibration factor to realize QAMS. At the same time, the external standard method was used to detect the contents of the six constituents, comparing the two methods to validate the accuracy and feasibility of the new method. Results No significant differences were found in the quantitative results of the six active constituents in Houttuyniae Herba determined by measured value and calculated value. The amounts of the six active constituents in the different parts of Houttuyniae Herba were found to be varied, chlorogenic acids and flavonoids mainly distributed in the leaves. Conclusion The active constituents ofHouttuyniae Herba mainly distribute in the leaves. The QAMS is feasible and accurate to evaluate the contents of the six active constituents in Houttuyniae Herba.

关 键 词：一测多评 相对校正因子 鱼腥草 绿原酸 芦丁 金丝桃苷 槲皮苷 异槲皮苷 

分 类 号：R286.2[医药卫生—中药学]