我国首次成功分离穿通支原体和发酵支原体的报告  

Report on First Isolation of Mycoplasma Penetrans and Mycoplasma Fermentans in China

作　　者：赵季文[1] 贾成梅 糜祖煌[3] 华咏[1] 徐萃瑜[1] 施素洁[4] 

机构地区：[1]南京东南大学公共卫生学院,南京210009 [2]江苏省卫生防疫站 [3]无锡市克隆遗传技术研究所 [4]苏州市卫生防疫站

出　　处：《疾病监测》2000年第11期405-407,共3页Disease Surveillance

摘　　要：本文报告了用病原学方法 ,了解我国AIDS患者和HIV感染者中是否存在穿通支原体、发酵支原体和梨支原体的感染。采集咽部分泌物及尿液 ,接种在SP - 4培养基作分离培养。阳性培养物用代谢抑制试验和聚合酶链反应等加以鉴定。从 2例AIDS患者的尿液中分离到 1株穿通支原体 ,7例HIV感染者尿液中分离到 1株发酵支原体。从病原学上首次证实 ,江苏地区AIDS患者和HIV感染者中存在穿通支原体和发酵支原体的感染 。The aim of this article was to research wether AIDS patients and HIV-infected individuals in our country contained mycoplasma penetrans (Mpe)、mycoplasma fermentans (Mf) and mycoplasma pirum (Mpi) infections. We cultured throat swabs and urine samples with the modified SP-4 culture medium for isolation. The positive culture was identificated with MIT and PCR. We isolated one Mpe strain from the urine of 2 AIDS patients and one Mf strain from the urine of 7 HIV-infected individuals. We were the first one to prove pathogenically that Mpe and Mf infections existed in AIDS patients and HIV-infected individuals in Jiangsu province, and it was necessary to pay attention to Mpe and Mf infections in AIDS patients.

关 键 词：穿通支原体 发酵支原体 分离 培养 

分 类 号：R375.1[医药卫生—病原生物学]