氯氰菊酯对小鼠脑细胞的氧化损伤及维生素E的抗氧化作用  

Oxidative damage of pesticide cypermethrin on mouse brain cells and the antioxidant role of vitamin E.

作　　者：马萍[1,2] 张忠杰[1] 焦铭[1] 单士刚[1] 武阳[2] 陈姣娥[1] 杨旭[2] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100 [2]华中师范大学生命科学学院环境生物医学实验室,湖北武汉430079

出　　处：《中国环境科学》2013年第7期1323-1327,共5页China Environmental Science

基　　金：湖北省教育厅科学技术研究项目(D20122803);国家自然科学基金重点项目(51136002);国家级大学生创新训练计划项目(201210927036)

摘　　要：以昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯加维生素E组,染毒组按10,20,40mg/kg 3个剂量水平,维生素E的剂量为100mg/kg,灌胃染毒小鼠7d.以脑组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量;以脑组织细胞测定DNA-蛋白质交联(DPC)系数.随着氯氰菊酯染毒剂量的升高,脑组织的ROS、MDA含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量≥20mg/kg时,ROS含量(841.3±100.34)、GSH含量[(12.54±1.316)nmol/L]和DPC系数(0.054±0.004)有显著差异(P<0.05);染毒剂量≥40mg/kg时,GSH含量[(10.51±1.545)nmol/L]有显著差异(P<0.05),ROS含量(1014.3±81.67)、MDA含量[(2.849±0.218)μmol/L]和DPC系数(0.079±0.005)有极显著差异(P<0.01).高剂量染毒加维生素E组与高剂量染毒组相比较,脑组织的ROS、MDA含量和DPC系数均有下降,GSH含量上升.脑组织的ROS(719.5±74.56)、GSH[(16.52±1.985)nmol/L]和DPC系数(0.055±0.005)有显著差异(P<0.05),MDA含量[(1.662±0.265)μmol/L]有极显著差异(P<0.01).较高剂量(≥20mg/kg)的氯氰菊酯能造成小鼠脑组织的氧化损伤,维生素E有抗氧化作用.Kunming mice were randomly grouped into six groups and orally administered with drugs daily for a week;the groups included one solvent control group,three cypermethrin groups,one high dose cypermethrin plus vitamin E protection group and one vitamin E group.The exposure doses of cypermethrin groups were 10,20and 40mg/kg respectively.Some brain tissues were then made into homogenates for the measurement of ROS（reactive oxygen species）,GSH（glutathione） and MDA（Malondialdehyde） contents.Meanwhile,DPC（DNA-protein Crosslink） coefficients were detected from brain cell suspension.The brain contents of ROS,MDA and DPC coefficients increased gradually in a dose-dependent manner,whereas GSH content decreased accordingly.In the exposure group with the dose of 20mg/kg,ROS（841.3±100.34）,GSH contents[（12.54±1.316）nmol/L] and DPC coefficients（0.054±0.004） were significantly higher than those of the control group（P0.05）;In the highest dose group（40mg/kg）,GSH content [（10.51±1.545）nmol/L]was significantly higher than that of the control group（P0.05）,the significance of differences of ROS（1014.3±81.67）、MDA contents[（2.849±0.218）μmol/L] and DPC coefficients（0.079±0.005） to the control group have all become extreme（P0.01）.However,compared to the high cypermethrin dose group,high dose cypermethrin plus vitamin E protection group had lower ROS（719.5±74.56）（P0.05）,MDA[（1.662±0.265）μmol/L]（P0.01） and DPC coefficients（0.055±0.005）（P0.05） levels,and higher GSH contents[（16.52±1.985）nmol/L]（P0.05）.Cyermethrin at certain doses（≥20mg/kg） can induce oxidative stress in mice brain,whereas vitamin E has antioxidant effects.

关 键 词：氯氰菊酯 活性氧 还原型谷胱甘肽 丙二醛 DNA-蛋白质交联 氧化损伤 

分 类 号：X503.22[环境科学与工程—环境工程] R994.60[医药卫生—毒理学]