CYP2C93,VKORC1-1639G>A和CYP4F2 rs2108622基因多态性对华法林剂量的影响  

Influence of CYP2C9*3,VKORC1-1639 G>A and CYP4F2 rs2108622 Genetic Polymorphisms on Warfarin Dosage

作　　者：闻武[1] 黄烽[1,2,3] 阳丽梅[1,4] 韩涛[1,2,3] 

机构地区：[1]福建医科大学省立临床医学院,福州350001 [2]福建省心血管病研究所 [3]福建省立医院心血管外科,福州350001 [4]福建省立医院药学部,福州350001

出　　处：《中国胸心血管外科临床杂志》2013年第4期375-380,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家"十二五"科技支撑计划项目(2011BA11B18)~~

摘　　要：目的探讨CYP2C9*3、VKORC1-1639 G>A、CYP4F2 rs2108622基因多态性对心瓣膜置换术后患者华法林剂量的影响。方法收集2011年11月至2012年8月期间在福建省立医院心血管外科接受手术治疗的患者,用聚合酶链式反应(polymerase chain reaction,PCR)-基因测序方法检测133例患者CYP2C9*3、VKORC1-1639G>A、CYP4F2 rs2108622基因多态性,按基因型分组,分别比较不同基因型间平均华法林剂量。结果 CYP2C9*3AA、AC、CC分别有127例、6例、0例,平均日华法林剂量分别为3.75 mg、2.13 mg,差异有统计学意义(P<0.05);VKORC1-1639 G>A GG、GA、AA分别有3例、32例、98例,平均日华法林剂量分别为6.00 mg、4.50 mg和3.00 mg,差异有统计学意义(P<0.05);CYP4F2 rs2108622 CC、CT、TT分别有67例、59例、7例,平均日华法林剂量分别为3.00 mg、3.75 mg和4.50 mg,差异有统计学意义(P<0.05)。结论 CYP2C9*3、VKORC1-1639 G>A、CYP4F2rs2108622基因多态性与个体华法林剂量差异有关。Objective To investigate the influence of CYP2C9 ＊3, VKORC1-1639 G 〉 A and CYP4F2 rs2108622 genetic polymorphisms on warfarin dosages of patients after heart valve replacement. Methods A total of 133 patients undergoing heart valve replacement in the Department of Cardiovascular Surgery of Fujian Provincial Hospital from November 2011 to August 2012 were included in this study. Polymerase chain reaction （PCR）gene sequencing was performed to detect CYP2C9 ＊3, VKORC1-1639 G 〉 A and CYP4F2 rs2108622 genetic polymorphism of these 133 patients. Patients were grouped according to their genotypes, and average warfarin dosages were compared between different genotype groups. Results The frequencies of CYP2C9 ＊ 3 AA, AC and CC were 127 patients, 6 patients and 0 patient respectively, and average daily warfarin dosages were 3.75 mg and 2.13 mg respectively which were statistically different between different CYP2C9 ＊ 3 genotypes （P 〈 0.05 ）. The frequencies of VKORC1-1639 G 〉 A GG, GA and AA were 3 patients, 32 patients and 98 patients respectively, and average daily warfarin dosages were 6.00 mg, 4.50 mg and 3.00 mg respectively which were statistically different between different VKORC1-1639 G 〉 A genotypes （P 〈 0.05）. The frequencies of CYP4F2 rs 2108622 CC, CT and TT were 67 patients, 59 patients and 7 patients respectively, and average daily warfarin dosages were 3.00 mg, 3.75 mg and 4.50 mg respectively which were statistically different between different CYP4F2 rs2108622 genotypes （P 〈 0.05）. Conclusion CYP2C9 ＊ 3, VKORC1-1639 G 〉 A and CYP4F2 rs2108622 genetic polymorphisms are associated with individual difference of warfarin dosages of patients after heart valve replacement.

关 键 词：CYP2C9＊3 VKORC1-1639G〉A CYP4F2 rs2108622 基因多态性 华法林 

分 类 号：R969.3[医药卫生—药理学]