呼吸道腺病毒感染患儿细胞因子及淋巴细胞亚群的研究  

Changes of lymphocyte subsets and cytokines secretion in children with respiratory ADV infection

作　　者：卢华君[1] 赵忠艳[1] 李云芳[1] 朱欢[1] 朱小舟[1] 陈小芳[1] 

机构地区：[1]温州医学院附属育英儿童医院,温州325027

出　　处：《中国人兽共患病学报》2013年第8期808-811,816,共5页Chinese Journal of Zoonoses

摘　　要：目的探讨呼吸道腺病毒(ADV)感染患儿的细胞因子水平及淋巴细胞亚群,及其在腺病毒感染发病与发展中的致病作用及意义。方法采用直接免疫荧光法检测呼吸道感染患儿咽拭子腺病毒抗原,结果为阳性者入选ADV感染组。采用流式细胞术(CBA)检测60例ADV感染患儿和30名正常对照儿童血清IL-2、IL-4、IL-6、IL-10、TNF、IFN-γ、IL-17A及外周血淋巴细胞亚群CD+3、CD+3CD+4、CD+3CD+8、CD+19、CD+16CD+56的百分比。结果 ADV感染患儿血清中的IL-2、IL-4、IL-6、TNF、IL-17A水平显著高于正常对照组(P<0.01);而血清中的IL-10、IFN-γ水平显著低于正常对照组(P<0.05);ADV感染患儿外周血CD+3、CD+3CD+4、CD+16CD+56水平及CD4/CD8比值明显低于正常对照组(P<0.05);而CD19+B淋巴细胞则明显高于正常对照组(P<0.01);外周血CD+3CD+8T淋巴细胞与正常对照组相比差异无统计学意义(P>0.05)。结论儿童呼吸道腺病毒感染会引起细胞因子水平及淋巴细胞免疫应答变化,提示免疫功能紊乱在发病及病情的发展中有重要作用。The aim of this study is to explore the changes of cytokines secretion and lymphocyte subsets in children with adenovirus （ADV） infection, and to investigate the relationship among ADV infection characteristics, prognosis, and individual immune response. Totally, 60 cases of hospitalized children with ADV antigen positive were selected as ADV infected group. The levels of cytokines secretion and lymphocyte subsets were detected in both ADV infected patients and 30 healthy children. The levels of serum IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ, and IL-17A were detected by cytometrie beads array. The percentages of lymphocyte subsets in peripheral blood, including CD3＋ , CD3＋ CD4＋ , CD3＋ CD8＋ , CD19＋ , and CD16＋ CD56＋, were detected by flow eytometry. The levels of serum IL-2, IL-4, IL-6, TNF, and IL-17A in ADV infected group were significantly higher than that in the normal group （P〈0.01）. And the levels of serum IL-10 and IFN-γ in ADV infected group were much lower than that in the normal group （P^0.05）. Compared with healthy children, the percentages of CD3＋ , CD3＋ CD4＋ , CD16＋CD56＋ and the ratio of CD4/CD8 were obviously decreased in ADV infected group （P〈0.05）. The percentage of CD19＋B, lymphocyte was sig nificantly higher than those in the normal group （P〈0.01）. There was no significant difference on CD3＋ CD8＋ T cells between two groups （P〉0.05）. It＇s indicated that the levels of cytokines in serum and lymphocyte subsets are unbalance due to the ADV infection. The immunologic response disorder may play an important role in the children with respiratory ADV infection.

关 键 词：腺病毒 淋巴细胞 细胞因子 流式细胞术 

分 类 号：R373[医药卫生—病原生物学]