清脂护肝方对非酒精性脂肪肝大鼠肝组织病理及纤维化指标的影响  

Effect of “Qingzhi Hugan Prescription”on liver tissue and fibrosis indicator in rats with nonalcoholic fatty liver disease

作　　者：张玲燕[1] 李民[1] 邵建国[1] 达坤林[1] 徐建中[1] 罗文明[1] 王世蓬[1] 罗琳[2] 周志玉[1] 

机构地区：[1]江苏省南通市第三人民医院,江苏南通226006 [2]南通大学医学院,江苏南通226001

出　　处：《上海中医药杂志》2013年第8期78-81,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：江苏省南通市社会发展科技计划资助项目(S5017;K11907)

摘　　要：目的探讨清脂护肝方对非酒精性脂肪肝大鼠肝组织病理变化及纤维化指标的影响。方法 76只SD大鼠除正常组10只外,其余大鼠以高脂饲料(88%普通饲料+2%胆固醇+10%猪油)喂养造模,12周后验证非酒精性脂肪肝大鼠造模成功。将造模成功的大鼠再随机分为模型组、阳性对照组、中药低剂量组、中药中剂量组和中药高剂量组,各组给予相应干预措施共6周。实验结束后进行大鼠肝组织半定量分析和光电镜下病理学观察,并检测大鼠肝纤维化指标,包括血清IV型胶原(CIV)、层粘连蛋白(LN)及肝组织匀浆CIV。结果与模型组比较,清脂护肝方各剂量组肝组织匀浆CIV、血清LN明显降低,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,清脂护肝方各剂量组脂肪变性程度明显减轻,差异有统计学意义(P<0.01)。清脂护肝方中剂量组、高剂量组脂肪变性积分较阳性治疗组明显降低,差异有统计学意义(P<0.05,P<0.01)。结论清脂护肝方具有良好的抗纤维化及脂肪变性作用,能改善实验性脂肪肝大鼠肝组织病理变化。Objective To observe the effect of ＂Qingzhi Hugan Prescription＂ on liver tissue and fibrosis indicator in rats with nonalcoholic fatty liver disease. Methods Ten in seventy-six rats were classified into normal group, the others were feed with high-fat diet for 12 weeks and the models were established. The models were randomized into model group, positive control group and Chinese medicine groups （ large, medium and small dose ） groups. Corresponding interventions for each group were given for 6 weeks. After the experiment, semiquantitative analysis on hepatic tissues and observed it by electron microscope. The CIV in serom, LN and CIV in liver tissue homogenate were detected. Results Compared with model group, the CIV in liver tissue homogenate and LN in serum of the Chinese medicine groups were decreased markedly （ P 〈 0.05 ,P 〈 0.01 ）. Compared with model group, the fatty degeneration of the Chinese medicine groups were decreased markedly （ P 〈 0.01 ）. The fatty degeneration of medium and large dose ＂Qingzhi Hugan Prescription＂ groups were lower than that of the positive treatment group significantly （ P 〈 0.05 P 〈 0.01 ）. Conclusion ＂Qingzhi Hugan Prescription＂ has a good effect on anti-fibrosis and fatty degeneration. It can improve the pathological changes in liver tissue of rats.

关 键 词：非酒精性脂肪肝 清脂护肝方 大鼠 病理 

分 类 号：R285.5[医药卫生—中药学]