断奶仔猪ACE2基因的克隆及遗传进化分析  

Cloning and phylogenetic analysis of angiotensin-converting enzyme 2(ACE2) gene of weaned piglets

作　　者：王珊珊[1] 杨维维[1] Amal 张源淑[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《畜牧与兽医》2013年第8期26-30,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30871838)

摘　　要：采用RT-PCR扩增和基因克隆技术鉴定断奶仔猪血管紧张素转化酶2(ACE2)基因序列,并与公布的猪以及其他物种的ACE2基因进行同源性比对。结果,成功克隆出了仔猪ACE2部分序列,该核苷酸序列与GenBank上公布的野猪ACE2核苷酸序列同源性达到98%,与欧洲牛的同源性为87.8%,与人的同源性为78.8%,与禽类(鸡)的同源性较低。遗传进化分析发现该扩增片段与牛的遗传距离最近,与鸡则处在完全不同的2个分枝上。氨基酸进化与基因遗传进化结果一致。本研究为仔猪ACE2的有效表达、生物学活性及其在苏姜猪中作用的研究奠定基础。The angiotensin-converting enzyme 2（ACE2） gene of weaned piglets was amplified by RT-PCR and sequenced.The results showed that the nucleotide sequence of ACE2 had 98%,87.8% and 78.8% of identities to those of Sus scrofa,Bos taurus and Homo sapiens published in GenBank,and had the lowest identity with Gallus gallus.Phylogenetic analysis showed the nearest genetic distance between piglet and Bos taurus,but a totally different two branches between piglet and Gallus gallus.The homologous analysis of amino acids showed the similar results as nucleotide.This study applied a basis for the investigation on ACE2 expression and biological activity.

关 键 词：血管紧张素转化酶2 断奶仔猪 基因克隆 遗传进化 

分 类 号：S852.23[农业科学—基础兽医学]