病原微生物实验室Ⅱ级生物安全柜防护性能评价  

EVALUATION ON THE PROTECTION PERFORMANCE OF CLASS Ⅱ BIOLOGICAL SAFETY CABINETS OF PATHOGENIC LABORATORY

作　　者：温占波[1] 胡凌飞[1] 李劲松[1] 杨文慧[1] 王洁[1] 李娜[1] 张柯[1] 殷喆[1] 董晓凯[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,国家生物防护装备工程技术中心,北京100071

出　　处：《中国消毒学杂志》2013年第8期703-705,709,共4页Chinese Journal of Disinfection

基　　金：国家科技支撑计划资助项目(编号:2008BAI62B05);"艾滋病和病毒性肝炎等重大传染病防治"重大专项课题(编号:2009ZX10004-501)

摘　　要：目的评价病原微生物实验室Ⅱ级生物安全柜的防护性能。方法采用安德森采样器采样方法和通过相关仪器,以粘质沙雷菌气溶胶为防护对象进行Ⅱ级生物安全柜防护性能评价。结果所评价的36台Ⅱ级生物安全柜下降气流的平均合格率为91.7%,流入气流合格率为43.7%,气流模型合格率为43.9%。人员保护合格率为72.2%,样品保护合格率为88.9%,交叉污染合格率为88.9%。结论所测试的Ⅱ级生物安全柜存在较大的气溶胶泄漏风险,对实验操作人员保护性能较差。Objective To evaluate the protection performance of class Ⅱ biological safety cabinets of pathogenic microorganism laboratory.Methods The protection performance was evaluated against Serratia marcescens aerosol by Andersen sampler and other special equipments.Results Of the 36 tested cabinets,the eligible rate of downflow velocity was 91.7%,that of inflow velocity,airflow smoke patterns,personnel protection,product protection and cross contamination was 43.7%,43.9%,72.2%,88.9% and 88.9% respectively.Conclusion There is great risk of aerosol leak from the tested biological safety cabinets and they do not provide enough personal protection effect to the lab personnel.

关 键 词：Ⅱ级生物安全柜 气溶胶泄露 病原微生物 防护性能 

分 类 号：R187.3[医药卫生—流行病学]