PKC在脑损伤后血脑屏障损害中的作用  

Effects of PKC on BBB in brain injury

作　　者：李良平[1] 郭希高[2] 徐如祥[1] 蔡颖谦[1] 

机构地区：[1]第一军医大学珠江医院神经外科,广州510282 [2]广东顺德市第一人民医院神经外科

出　　处：《脑与神经疾病杂志》2000年第5期262-264,共3页Journal of Brain and Nervous Diseases

摘　　要：目的:探讨PKC在脑损伤后血脑屏障损害中的作用。方法:雄性SD大鼠60只,随机分为正常对照组、假手术组、脑损伤组和H7治疗组。治疗组于伤后0.5h腹腔注射PKC阻断剂H7 1mg/kg,12h重复注射一次,荧光显微镜定性观察,分光光度计定量检测伊文氏兰渗出。结果:脑损伤后伊文氏兰明显渗出,治疗组渗出明显减少。结论:应用PKC阻断剂H7能明显阻止血脑屏障开放,表明PKC在脑损伤后血脑屏障损害中起重要作用。Objective: Aim To explore the effects of PKC on BBB damage in brain in jury. Methods: 60 male SD rats were devided into 4 of groups included control, Sham-operation, injury only and injury . with H7-treatment. The rats in the treatment group were peritoneally administered H7 (2mg/kg) at 0. 5h and 12h after brain injury. The permeation of Evans blue was observed qualitatively with an epifluorescence and quantitatively with a spectrophotometer. Resul ts: Evans blue significantly in the injured group while significantly decreases in treatment group. Conclusion: H7, a PKC blocker can prevent BBB opening significantly, these results indicate that the PKC take a important role on BBB damage in brain injury.

关 键 词：脑损伤 血脑屏障 蛋白激酶C 病理 

分 类 号：R651.150.2[医药卫生—外科学]