食管基底细胞样鳞状细胞癌免疫组化研究  

Immunohistochemical study of esophageal basaloid squamous cell carcinoma

作　　者：黄致治[1] 吴贤英[1] 梁英锐[1] 李乔山[1] 沈健[1] 

机构地区：[1]汕头大学医学院病理教研室,广东省汕头市515031

出　　处：《世界华人消化杂志》2000年第10期1097-1100,共4页World Chinese Journal of Digestology

摘　　要：目的了解食管基底细胞样鳞状细胞癌(BSC)的生物学特点.方法 BSC 8例,用 CK4,CK18,CK19,EMA,CEA,SMA,S-100,LN,ColⅣ,NSE,PCNA,p53等12种抗体,进行免疫组化检测,用常规 ABC 法.结果 BSC 的两种细胞成分,基底样细胞(BC)和鳞状细胞(SC)对上述抗体反应截然不同.BC 绝大部分病例(7例)专一地对 CK19抗体呈阳性反应,而对其他4种上皮性抗体呈阴性;BC 有4例对α-SMA 及 S-100肌性抗体呈阳性;BC 巢内玻璃样物对 LN 及 ColⅣ呈阳性反应;BC 有较高的 PCNA 指数,平均54%.相反,SC 全部病例均对 CK4,CEA,EMA 等上皮性抗体呈阳性,而对 CK19,SMA,S-100阴性.PCNA 指数也较低,平均25%.结论食管 BSC 为多向分化的高度恶性肿瘤,BC 代表向基底细胞和肌上皮方向分化,其增殖活性强,而 SC 代表向典型的鳞状细胞分化.AIM To probe the biological features of basaloid- squamous carcinoma (BSC) of esophagus. METHODS Using ABC method to perform immunohistochemistry for 8 BSC cases by different antibodies:CK4,CK18,CK19,EMA,CEA,SMA,S-100, LN,ColⅣ,NSE,PCNA and P53. RESULTS BSC had 2 different cell components:basaloid cell (BC) and squamous cell (SC),and they had completely different reactions to the above antibodies.In the majority of BC (7 cases),they had positive reaction to CK19 specifically,while to the other 4 epithelial antibodies they had negative results.There were 4 cases of BC in which SMA and S-100 (antibodies related to muscle cells) were positive.The hyaline like substance inside the cancer cell nests was LN and ColⅣ positive.BC had rather high PCNA index (54% on average),while SC had lower PCNA index (25% on average).In SC the epithelial related antibodies CK4,EMA,CEA were positive,while CK19,SMA,S-100 were negative. CONCLUSION BSC of esophagus is a malignant tumor with multiple-direction differentiation.The BC cell component represents the differentiation toward both basal cell and myoepithelial cell;these cells have strong proliferation activity and invasive potential,while the SC component represents the differentiation toward typical squamous cells.

关 键 词：食管肿瘤 免疫组织化学 基底细胞样鳞状细胞癌 

分 类 号：R735.1[医药卫生—肿瘤]