无浆血小板制备的研究  

Study on no-plasma platelet preparation

作　　者：李宁[1] 叶萍[1] 毕晓琳[1] 

机构地区：[1]大连市血液中心,辽宁大连116001

出　　处：《临床血液学杂志（输血与检验）》2013年第4期515-517,520,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:比较无浆血小板、洗涤血小板和机采少白细胞血小板在血小板回收率、血浆蛋白清除率、血小板体外激活方面的差异,为临床出现输血不良反应的患者提供新的治疗途径。方法:机采血小板时改变机器设置参数,不采集血浆,制备无浆血小板为实验组,对照组为机采少白细胞血小板和洗涤血小板,分别检测无浆组、对照组、洗涤组血小板的计数、平均体积、大型血小板比率,pH值及血浆蛋白含量,通过对ADP激活前后CD62P、PAC-1的表达率检测3组血小板的活性。结果:无浆组血浆蛋白含量和洗涤组比较差异无统计学意义(P>0.05);血小板活性与对照组比较差异无统计学意义(P>0.05);洗涤组血小板计数与对照组、无浆组比较差异有统计学意义(均P<0.01);洗涤组血小板回收率平均为78%,对照组MPV、P-LCR、pH与洗涤组、无浆组比较差异有统计学意义,但3组结果均在正常范围内;洗涤组CD62P、PAC-1的再表达率与对照组,无浆组比较差异有统计学意义(P<0.01)。结论:无浆血小板可作为一种高效、低不良反应的制剂为临床广泛应用。Objective：To compare the differences of platelet recovery,content of plasma protein and platelet activation in vitro between no-plasma platelet,washed platelet and apheresis platelet leukoreduced,and offer a new clinical method for patients with transfusion adverse reaction.Method：Experiment group was no-plasma platelet prepared from random donor apheresis collections by changing the parameter settings.Control groups were consisted of apheresis platelet leukoreduced and washed platelet.Among the three groups,PLT counts,MPV,P-LCR,pH,plasma protein,and the expression of PAC-1 and CD62P before and after activated by ADP were assayed.Result：There was no significant difference between no-plasma group and washed group in the content of plasma protein（P〉0.05）.The activation of platelet in the no-plasma group had no significant difference from the control group（P〉0.05）.The platelets counts in washed group showed significant difference from the other two groups and the mean platelet recovery was 78%.MPV,P-LCR,pH in control group had significant difference from the other two groups but they were all in the normal range.The re-expression of the CD62P and PAC-1 in the washed group showed great significant difference from the other two groups（P〈0.01）.Conclusion：No-plasma platelet could be used in clinical patients because of its high efficiency and low transfusion adverse reaction.

关 键 词：无浆血小板 洗涤血小板 机采少白血小板 血小板活性 

分 类 号：R558[医药卫生—血液循环系统疾病]