卷烟烟气暴露对小鼠肺巨噬细胞吞噬功能和调节性T细胞的影响  

Effect of cigarette smoke exposure on phagocytic function of lung macrophage and Treg cells in mice

作　　者：李若曦[1,2] 刘姜瑾[3] 熊珊珊[2] 胡旺顺 杨陟华[2] 杜丽[2] 徐龙[2] 刘坤璐[2] 钟科军 潘秀颉[2] 朱茂祥[1,2] 

机构地区：[1]安徽医科大学研究生院,合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]湖南中烟工业有限责任公司技术中心,长沙410007

出　　处：《安徽医科大学学报》2013年第9期1014-1018,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81001219)

摘　　要：目的观察卷烟烟气对小鼠肺泡巨噬细胞(AM)的影响,探讨调节性T(Treg)细胞与AM吞噬功能的关系。方法 BALB/c小鼠经卷烟烟气暴露1、2、3个月后处死,姬姆萨染色计数支气管肺泡灌洗液(BALF)中AM数目,吞噬D大肠杆菌(E.coli)检测AM吞噬功能,流式细胞仪检测外周血及BALF中Treg细胞的比例,蛋白液体悬浮芯片技术检测BALF炎性细胞因子。结果小鼠暴露卷烟烟气1个月,BALF中AM数显著增高,AM吞噬功能和BALF中Treg细胞比例显著降低,炎性细胞因子白介素(IL)-1β、单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎症蛋白-1α(MIP-1α)含量增高,IL-10量减少;暴露2个月,AM数量及其吞噬功能和BALF中Treg细胞比例均显著降低,MCP-1及MIP-1α含量增高,IL-1β、IL-10含量减少;暴露3个月,除IL-10含量增高外,其他观察指标与对照组比较,差异无统计学意义。结论卷烟烟气对小鼠AM功能有影响,Treg细胞与AM功能关系密切,并受炎性细胞因子调节。Objective To investigate the effect of cigarette smoke exposure on phagocytic function of alveolar macrophage（AM） and regulator T（Treg） cells in mice.Methods The BALB/c mice were executed on 1,2 and 3 month after exposure to cigarette smoke.The AM were collected to count and test phagocytosis functions by swallowing E.coli.The ratio of CD4＋CD25＋Foxp3＋Treg cell in peripheral blood and bronchoalveolar lavage fluid（BALF） were analyzed by flow cytometry.The cytokines in BALF were examined by Bio-plex System.Results Compared with control group,1 month after cigarette smoke exposure,the number of AM and the concentration of IL-1β,MIP-1 and MCP-1α increased,and phagocytic function of AM,the ratio of BALF CD4＋CD25＋Foxp3＋Treg cells in CD4＋T cells and the concentration of IL-10 decreased.2 months after cigarette smoke exposure,the number and phagocytic function of AM,the ratio of BALF CD4＋CD25＋Foxp3＋Treg cells in CD4＋T cells and the concentration of interleukin（IL）-1β reduced,and the concentration of monocyte chemotactic protein-1（MIP-1） and macrophage inflammation protein-1α（MCP-1α） increased.However,3 months after cigarette smoke exposure,all indicators showed no statistical difference from normal group other than the concentration of IL-10（increased）.Conclusion There are obvious effects of cigarette smoke exposure on phagocytic function of mice macrophage and Treg cell,and some inflammatory factors are probably concerned with the process.

关 键 词：卷烟烟气 肺泡巨噬细胞 调节性T细胞 炎性细胞因子 

分 类 号：R114[医药卫生—卫生毒理学] R563.9[医药卫生—公共卫生与预防医学]