人三叶因子3对烧伤小鼠胃黏膜的保护作用研究  

Protective effect of recombinant hTFF3 on gastric mucosal lesions of burned mice

作　　者：孙勇[1] 王良喜[1] 毛学飞[1] 邓向东[1] 潘晓峰[1] 吴杭庆[1] 张盼[1] 张方[1] 

机构地区：[1]中国人民解放军第九七医院烧伤整形科,徐州221004

出　　处：《安徽医科大学学报》2013年第9期1019-1022,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81100252);南京军区医学科研课题重点项目(编号:09Z007);中国人民解放军第九七医院院管课题(编号:YN2011010)

摘　　要：目的观察基因重组人三叶因子3(hTFF3)对严重烧伤所致胃黏膜损害的保护作用。方法采用30%体表面积Ⅲ°烧伤小鼠作为模型。将小鼠随机分成正常对照(A)组、烧伤对照(B)组和hTFF3治疗(C)组,C组伤后1 h开始灌胃hTFF3,剂量为1 mg/kg,B组灌胃等量生理盐水,各组每天灌胃1次,连续5 d。观察烧伤后胃黏膜损伤指数、胃黏膜大体形态学改变、病理学改变,以及病死率。结果烧伤小鼠每天给予1 mg/kg的hTFF3灌胃,胃肠黏膜受损程度显著降低,胃黏膜病理改变明显减轻,B组以出血、坏死和溃疡为主,C组以充血、水肿为主,小鼠5 d病死率有所下降(27.3%、45.6%)。结论重组hTFF3对烧伤后胃肠道损伤具有明显的治疗作用,能促进受损黏膜屏障的重建。Objective To explore the effect of recombinant human trefoil factor 3（hTFF3） on burn-induced gastric injury in mice.Methods Kunming mice were inflicted with 30% total body surface area full thickness burns and randomly divided into three groups： normal control（A）,burned（B）,burned and hTFF3 treatment（C）.After injury,mice were administrated by hTFF3 orally once daily.Injury index,pathomorphological changes,death rate and so on were determined on 0.5,1,3 and 5 post burn days（PBDs）.Results hTFF3 treatment（1 mg / kg） significantly decreased the injury index,alleviated pathological damage and reduced death rate in comparison with the control group.Conclusion hTFF3 enhances the healing effect on the injury in gastrointestinal tract of mice.

关 键 词：三叶因子3 烧伤 胃黏膜 

分 类 号：R644[医药卫生—外科学]