人MRG15在哺乳动物细胞中表达与定位的初步研究  

A preliminary study of the expression and localization of human MRG15 in mammalian cells

作　　者：姜天鹏[1] 潘林鑫[1] 赵健[1] 乔正[1] 李春雨[1] 张珊静[1] 李克娟[1] 范礼斌[1] 

机构地区：[1]安徽医科大学生命科学学院生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2013年第9期1022-1025,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学重点科研项目(编号:KJ2010A187);安徽省自然科学基金(编号:11040606M170);安徽医科大学博士科研基金(编号:XJ2005004)

摘　　要：目的研究细胞周期调控蛋白(MRG15)在哺乳动物细胞中的表达与定位。方法以含人MRG15全长cDNA序列的质粒为模板,PCR扩增,构建MRG15真核表达载体,分别利用Western blot法和免疫荧光法检测其在哺乳动物细胞株中的表达与定位。结果 Western blot法检测结果表明MRG15在哺乳动物细胞中能有效地表达,免疫荧光法结果显示MRG15主要分布在细胞核。结论 MRG15蛋白主要在细胞核中表达,为进一步了解MRG15的功能奠定了一定基础。Objective To investigate the location and expression of the cell cycle regulatory protein（MRG15） in mammalian cells.Methods The full length cDNA sequence of MRG15 from plasmid was used to amplify with PCR,to conduct the eukaryotic expression vector of MRG15.Immunofluorescence and Western blot were adopted to detect the location and expression of MRG15 in the cell strain.Results Western blot results showed effective expression in the cells.Besides,the results of immunofluorescence indicated that MRG15 was located in cell nucleus.Conclusion The expression of MRG15 protein is localized in the nucleus,which lays foundation to further study of the function of MRG15.

关 键 词：MRG15 蛋白表达 免疫荧光 

分 类 号：R341[医药卫生—基础医学] R394.2