昆虫几丁质酶的进化与保守性质的比较基因组学研究  

Evolution and consensus exploration of insects’ chitinase by comparative genomics

作　　者：黄海云[1] 张屹[2] 万张勇[3] 

机构地区：[1]河北科技大学图书馆,河北石家庄050018 [2]河北科技大学理学院,河北石家庄050018 [3]河北科技大学研究生学院,河北石家庄050018

出　　处：《河北科技大学学报》2013年第4期350-354,共5页Journal of Hebei University of Science and Technology

基　　金：国家自然科学基金(11171088);河北省自然科学基金(A2011208002)

摘　　要：昆虫几丁质酶家族属于第18家族糖苷键水解酶,主要参与昆虫蜕皮、细胞增殖和免疫等生理过程。在7个物种基因组数据中通过BLASTP和PSI-BLAST的方法获得131条chitinase和chitinase-like的同源蛋白质,利用隐马尔科夫算法,找到了几丁质蛋白的保守模体区域。通过进化树分析,将所选的蛋白分为9组,与糖苷水解酶18催化结构域和几丁质结合结构域的特性一致。通过PAML软件计算出几丁质酶的正向选择位点,发现了豌豆蚜虫中的A.pisum xp003243674.1为一条新的几丁质酶蛋白。对深入研究昆虫几丁质酶结构与功能很有帮助。Insects＂ chitinase family belongs to the 18th family glycosidic bond hydrolytic enzyme, mainly involved in the physio- logical processes of molting, cell proliferation and immunization. In this paper, from the seven species＂ genome data, 131 ho- mologous chitinase and chitinase-like proteins are obtained by BLASTP and PSI-BLAST. The consensus motif sequences is ex- tracted by HMM algorithm. Evolution tree analysis classifies these 131 proteins into 9 groups, which are consistent with the characteristics of glycoside hydrolase 18 （Glyco_18） catalytic domain and chitin binding domain. Moreover, by PAML soft- ware, the positive selection sites for chitinase are calculated and a new chitinase A. pisum xp003243674. I is found. This paper will be helpful in further research of chitinase structure and function.

关 键 词：几丁质酶 同源蛋白 进化树 模体 正向选择位点 

分 类 号：Q781[生物学—分子生物学]