通络定眩方对椎动脉型颈椎病兔血液流变学、ET、NO的影响  

Effects of Tongluo Dingxuan decoction on hemorheology,ET,NO of rabbits with vertebral artery type cervical spondylosis

作　　者：胡兆斌[1] 傅强[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《实用临床医药杂志》2013年第15期1-4,共4页Journal of Clinical Medicine in Practice

摘　　要：目的探讨通络定眩方对椎动脉型颈椎病模型兔一氧化氮(NO)、血浆内皮素(ET)、血液流变学影响,为中医药在椎动脉型颈椎病的实验研究提供有价值的内容。方法参照成都中医药大学临床医学院朱明双的注射硬化剂法建立椎动脉型颈椎病家兔模型,健康普通家兔40只,分为空白组、模型组、颈复康治疗组以及通络定眩方治疗组,每组10只,对通络定眩方治疗组模型兔给予通络定眩方灌胃,颈复康治疗组模型兔给予颈复康颗粒混悬液灌胃,另2组以等量生理盐水灌胃,实验前后检测模型兔血浆内皮素(ET)、氧化亚氮(NO),实验后检测血液流变学并以此作为疗效评价指标,统计分析各组在上述指标方面的差异,并分析比较。结果模型组全血高切、中切、低切黏度明显高于空白组、颈复康组、通络定眩方组(P<0.05),而颈复康组、通络定眩方组比较差异无统计学意义(P>0.05),提示通络定眩方改善家兔实验性椎动脉型颈椎病的血液流变学的作用与颈复康颗粒相当;给药后模型组血浆ET含量明显高于空白组、颈复康组、通络定眩方组,而颈复康组与通络定眩方组比较,前者ET减少量小于后者(P<0.05);给药后模型组血清NO明显低于空白组、颈复康组、通络定眩方组(P<0.05),通络定眩方和颈复康比较差异无统计学意义(P>0.05),提示通络定眩方在增加血清NO、降低血浆ET方面有较好效果,甚至对后者的作用优于颈复康颗粒。结论①通络定眩方及"颈复康颗粒"均具有良好的实验效果,可改善椎动脉供血,调整内皮细胞功能;②在降低ET方面通络定眩方略优于"颈复康颗粒";③在改善血液流变学及升高NO方面通络定眩方与"颈复康颗粒"相当。Objective To investigate the effects of Tongluo Dingxuan decoction on nitric oxide （NO）, plasma endothelin （ET）, hemorheology of rabbits modes with vertebral artery type cervical spondylosis, and provide reference to the treatment of cervical vertebral artery disease with traditional Chinese medicine. Methods According to injection sclerotherapy by Zhu Mingshuang, a professor from Chengdu University of Traditional Chinese Medicine, vertebral artery type cervical spondylosis rabbit model was established. Forty healthy ordinary rabbits were divided into control group, model group, Jingfukang group and Tongluo Dingxuan decoction group, 10 rabbits in each group. Tongluo Dingxuan decoction group received Tongluo Dingxuan by means of gavage suspen- sion, Jingfukang group received Jiangfukang particles, and the other two groups received normal saline gavage. Before and after the experiment, ET and NO levels were measured. Hemorheology was measured after experiment. The differences of the four groups in terms of the above tioned indexes were statistically analyzed and compared. Results The high - shear, middle and low - shear viscosities of whole blood were significantly higher in the model group than - shear in the rest three groups （P〈 0.05）. The differences between Jingfukang group and Tongluo Dingxun group showed no statistically significant difference （P 〉0.05）, suggesting that Tongluo Dingxuan shared similar effect in improving hemorheology with J Iangfukang particles. After treatment, the amount of plasma ET was significantly higher in the model group than in the rest three groups. The amount of ET was lower in Jingfukang group than in Tongluo Dingxuan group （ P 〈 0.05）. After treatment, the level of serum NO was significantly lower in the control group than in the rest three groups （P 〈 0.05）. The difference between Tongluo Dingxuan decoction and Jingfukang particles were not statistically significant （P 〉0.05）, suggesting that Tongluo Dingxuan decoction were more effective i

关 键 词：椎动脉型颈椎病 中药汤剂 通络定眩方 实验研究 

分 类 号：R681[医药卫生—骨科学]