类风湿性关节炎患者血清白介素-33、肿瘤坏死因子-α、C反应蛋白的水平变化及意义  

Changes and significance of interleukin-33,tumor necrosis factor-α and C reaction protein levels in patients with rheumatoid arthritis

作　　者：黄文辉[1] 陈瑞林[1] 曾颖瑜[1] 林泽英[1] 

机构地区：[1]广州医科大学附属第二医院风湿免疫科,广东广州510260

出　　处：《实用临床医药杂志》2013年第15期5-7,共3页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11321092)

摘　　要：目的探讨类风湿性关节炎患者血清白介素-33(IL-33)、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)的水平变化及意义。方法 50例类风湿性关节炎(RA)患者为RA组,根据中华医学会风湿病学分会制定的RA活动期判定标准分-为活动期RA组和非活动期RA组,每组25例。另选择本院体检中心接受体检的健康成年人30例为对照组。各组患者清晨空腹采集肘静脉血5 mL,分离血清,免疫比浊法测定血清中CRP水平,双抗体夹心酶联免疫吸附实验(Elisa)检测血清中IL-33、TNF-α水平。比较各组血清中IL-33、TNF-α、CRP水平;分析RA组患者血清IL-33与TNF-α水平的相关性,及血清IL-33、TNF-α水平与CRP水平间的相关性。结果 RA组患者(活动期/非活动期)血清中IL-33、TNF-α、CRP水平均明显高于对照组;活动期RA组患者血清IL-33、TNF-α、CRP水平均明显高于非活动期RA组患者。RA组患者血清IL-33与TNF-α水平呈显著正相关;IL-33、TNF-α与CRP水平均呈显著正相关。结论检测RA患者血清IL-33、TNF-α、CRP水平对观察其病情变化具有重要的临床价值。Objective To explore the changes and significance of interleukin-33 （IL-33）, tumor necrosis factor-α（TNF-α） and C reactive protein （CRP） levels in patients with rheumatoid arthritis （RA）. Methods Fifty patients with RA were divided into active-stage RA group and non -active stage RA group according to RA active-stage criterion of Chinese Rheumatology Association, 25 cases in each group. Thirty healthy people that underwent physical examination in the medical center of our hospital were selected as control group. Collection of 5 mL blood was made from fasting patients in each group in the morning, followed by serapheresis. Serum CRP level was detected by immunoturbidimentry, IL-33 and TNF-α level were examined by double antibody sandwich enzyme linked immunosorbent assay （Elisa）. Then serum IL-33, TNF-α and CRP levels in each group were compared, while the correlations between IL-33 and TNF-α as well as between IL-33, TNF-α and CRP in RA group were analyzed. Results Serum IL-33, TNF-α and CRP levels in the RA group were markedly higher than those in the control group. They were also higher in active-stage RA group than in non-active-stage RA group. Serum IL-33 level was in positive correlation with TNF-α, and IL-33 and TNF-α were positively related to CRP level in the RA group. Conclusion Serum IL-33, TNF-α and CRP levels in RA group are of great clinical importance in observing RA pathogenic conditions.

关 键 词：类风湿性关节炎 血清白介素-33 肿瘤坏死因子-Α C反应蛋白 

分 类 号：R593.22[医药卫生—内科学]