人参对大鼠创面及血清TNF-α、IL-1β、TGF-β_1的影响  

Effect of Ginseng on Wound and Serum TNF-α,IL-1β,TGF-β_1 of Rats

作　　者：黄治官[1] 张晓辉[1] 李国君[1] 张林挺[1] 谢单洁 李裕和[1] 

机构地区：[1]广州体育学院,广东广州510500 [2]广东省重竞技体育训练中心,广东广州515000

出　　处：《河南中医》2013年第8期1250-1252,共3页Henan Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(编号:S2011010003089)

摘　　要：目的:研究人参对大鼠创面愈合的作用及血清TNF-α、IL-1β、TGF-β1的影响。方法:30只SPF级8周龄SD大鼠随机均分为健康对照组、模型安静组、模型人参组,雌雄各半。麻醉及消毒后切除大鼠背部2 cm方形全层皮肤造模。伤后第3天开始人参灌胃,持续14 d。常规观察和测量创面愈合情况;伤后第10天、第17天断尾取血备血清,ELISA法测IL-1β、TNF-α、TGF-β1。结果:人参使皮肤创面愈合时间加快(2±0.5)d,愈合质量较好;伤后第10天、第17天人参组血清TNF-α、IL-1β水平均明显低于模型安静组(P<0.05);TGF-β1水平在第10天明显高于模型安静组(P<0.05),第17天与模型安静组比较无显著性差异(P>0.05)。结论:人参可能通过改善创面炎症免疫反应及TGF-α、IL-1β、TGF-β1相关通路促进创面愈合。Objective： To study the effect of ginseng on wound healing and serum TNF-α,IL-1β,TGF-β1 of rats.Methods： 30 SPF SD rats aged 8 weeks were randomly divided into control group,model of quiet group,model of ginseng group,half male and half female.2cm squares of rat back skin were cut off to make model after anesthesia and disinfection.On the 3rd day after injury,the rats were given gastric perfusion of ginseng for 14D.Regular observation and measurement of wound healing.On the 10th day and 17th day after injury,the tail was cut to take blood serum;ELISA method was used to test IL-1β,TNF-α,TGF-β1.Results： Ginseng made the healing time of skin wound increase 2 ± 0.5 d,the healing quality was better;On the 10th day and 17th day after injury,serum levels of IL-1β,TNF-α in ginseng group were significantly lower than those of model group（P0.05）;TGF-β1 level on the 10th day was significantly higher than that in model group（P0.05）,on the 17th day,there was no significant differences（P0.05） compared with the model group.Conclusion： Ginseng may promote the wound healing by improving the wound inflammatory immune response and the relative pathways of TGF-α,IL-1β,TGF-β1.

关 键 词：人参 创面愈合 IL-1Β TNF-α TGF-Β1 SD大鼠 

分 类 号：R285.5[医药卫生—中药学]