重庆市汉族哮喘儿童α_(1)-抗糜蛋白酶表型分析研究  

Study of phenotypes of α_(1)antichymotrypsin in Han asthmatic children in Chongqing

作　　者：尹晓娟[1] 李为明[2] 奚敏[2] 

机构地区：[1]第三军医大学西南医院儿科,重庆400038 [2]第三军医大学新桥医院儿科,重庆400037

出　　处：《免疫学杂志》2000年第6期448-450,共3页Immunological Journal

基　　金：国家自然科学基金资助项目(39570749)

摘　　要：目的探讨α_1-糜蛋白酶 (α_1-ACT)不同表型在哮喘发病中的作用。方法应用聚丙烯酰胺凝胶等电聚焦免 疫固定技术,对95例哮喘儿童和180例健康儿童血清α_1-抗糜蛋白酶的蛋白表型进 行了检测。结果哮喘组和儿童对照组血清α_1-抗 糜蛋白酶均存在3种表型,哮喘组与儿童对照组比较,α_1-ACT Ⅰ型表型和Ⅱ型表 型分布2组间均无显著性差异(P>0.05);α_1ACT Ⅲ型表型分布哮喘 组明显高于对照组(P<0.01)。结论α_ 1ACT Ⅲ型表型增加了重庆市汉族儿童患哮喘的危险性。Objective To explore the pathogenetic role of α_1- ACT phenotypes in childhood asthma.Methods Phenotypes of α_1- ACT have been determined by isoelectric focusing on the polyacrylamide gel foll owed by immunofixation,the phenotypes of α_1-ACT were investigated using this method in asthmatic and control groups.Results Three phenotypes of α_1-ACT were found in two groups.The re was no difference in phenotype Ⅰ and phenotype Ⅱ of α_1-ACT between these two groups(P>0..05).Phenotype Ⅲ of α_1-A CT in asthma group showed a higher prevalence than normal control group(P<0.01 ).Conclusion This study provides the evidence that phenotype Ⅲ of α_1-ACT increases the risk of developing asthma during childhood.

关 键 词：儿童哮喘 α_(1)-抗糜蛋白酶 表型 

分 类 号：R562.25[医药卫生—呼吸系统]